Piperazine-1,4-bis(2-ethanesulfonic Acid) Disodium Salt is a chemical compound widely used as a buffer in biological research applications. It plays a crucial role in maintaining stable pH levels in various experimental setups, ensuring that biochemical reactions occur under controlled conditions. This buffer is particularly valued for its ability to resist changes in pH, making it essential in experiments that require precise environmental control. Its versatility allows it to be used across a wide pH range, which is critical in many laboratory processes.
One of the key properties that make this compound a preferred choice is its chemical stability and resistance to degradation. This ensures that the buffer remains effective over extended periods, reducing the risk of contamination and maintaining the accuracy of experimental results. Additionally, its solubility in water and compatibility with a variety of biological systems make it a reliable component in both routine and specialized laboratory workflows. These characteristics contribute to its widespread use in research settings where consistency and reliability are paramount.
In Indonesian laboratories, this compound is commonly used in molecular biology, biochemistry, and pharmacology research. It is frequently employed to prepare buffer solutions for enzyme activation, protein testing, and chemical analysis. Its availability supports the growing demand for high-quality reagents in local research institutions, enabling scientists to conduct accurate and reproducible experiments. The compound's role in maintaining pH stability is vital for the success of many biological and chemical investigations.
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- Molecular biology experiments benefit from this buffer due to its ability to maintain stable pH during DNA and RNA manipulation.
- Biochemical assays rely on its consistent performance in enzyme activity tests and protein purification processes.
- Pharmacological studies use it to create controlled environments for drug testing and receptor binding analysis.
- Cell culture applications require precise pH control, which this buffer provides for optimal cell growth and viability.
- Analytical chemistry procedures depend on its stability and solubility for accurate and reproducible results in titration and spectroscopy.
| Brand | TCI |
|---|---|
| CAS number | 76836-02-7 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Biological Buffers |
| Pack sizes | Available in standard laboratory quantities |
| Physical form | Powder |
| Storage | Store in a cool, dry place away from direct sunlight |
This compound should be stored in a cool, dry environment to maintain its chemical stability and prevent degradation. It is recommended to keep it in airtight containers to avoid moisture absorption, which can affect its performance. Due to its hygroscopic nature, it is important to ensure that the storage area is well-ventilated and free from contaminants. Avoid exposure to extreme temperatures or direct sunlight, as these conditions may compromise its effectiveness. Always handle the material with appropriate personal protective equipment, such as gloves and safety goggles, to ensure safe laboratory practices. Proper storage and handling are essential to maintaining the integrity and reliability of this buffer in research applications.
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