• 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] (CAS 16052-06-5) - TCI H0576 - AMI Scientific
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TCI

CAS 16052-06-5

4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] TCI H0576

4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research] TCI H0576

Chemical Identity

Other names
HEPPS · 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic Acid
CAS No.
16052-06-5
Formula
C9H20N2O4S
Molecular weight
252.33 g/mol
Purity
>98.0%(T)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid
SMILES
C1CN(CCN1CCCS(=O)(=O)O)CCO
InChIKey
OWXMKDGYPWMGEB-UHFFFAOYSA-N
MDL
MDL00006160
PubChem
CID 85255
Rp 1.565.550,00
Rp 1.565.550,00
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4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid, commonly known as HEPES, is a vital biochemical used as a buffer in various biological research applications. This compound is essential in maintaining a stable pH environment in experimental solutions, which is critical for the accuracy and reproducibility of laboratory procedures. HEPES is widely utilized in life sciences for its ability to resist pH changes, making it indispensable in maintaining the integrity of biological systems during experiments. Its role extends beyond simple buffering, as it supports the stability of proteins, enzymes, and cells in controlled environments.

HEPES is preferred due to its non-toxic nature, lack of odor, and stability under a wide range of conditions. These properties ensure that it is safe to handle and suitable for use in sensitive biological applications. Its high water solubility allows for easy incorporation into various solutions, simplifying the preparation of experimental media. Additionally, HEPES remains effective across a broad pH range, making it a versatile choice for different experimental setups. Its inert nature also minimizes interference with biological processes, enhancing the reliability of experimental results.

In Indonesian laboratories, HEPES is extensively used in molecular biology, pharmacology, and biotechnology research. It is a key component in the preparation of culture media, enzyme reaction solutions, and cell culture environments. Its widespread availability and consistent performance support a variety of experimental protocols, contributing to the advancement of scientific research in the region. The reliability and effectiveness of HEPES make it an essential tool for researchers working in both academic and industrial settings.

  • HEPES is used in cell culture media to maintain a stable pH environment, ensuring optimal cell growth and viability.
  • It is essential in enzyme reaction solutions, where pH stability is crucial for maintaining enzymatic activity and reaction efficiency.
  • HEPES supports PCR (Polymerase Chain Reaction) protocols by providing a consistent pH buffer, which is necessary for DNA amplification.
  • It is commonly used in protein purification processes to preserve protein structure and function during isolation and analysis.
  • HEPES is integral in the preparation of buffer solutions for electrophoresis, ensuring accurate separation of nucleic acids and proteins.

Brand TCI
CAS number 16052-06-5
Molecular formula —
Purity —
Category Life Science > Biochemicals and Reagents > Biological Buffers
Pack sizes Available in various quantities as per standard laboratory requirements
Physical form White crystalline powder
Storage Store in a cool, dry place away from direct sunlight

HEPES should be stored in a cool, dry place, away from direct sunlight and sources of heat. It is recommended to keep the product in a tightly sealed container to prevent moisture absorption and contamination. Due to its non-toxic nature, HEPES is generally safe to handle, but standard laboratory safety precautions should still be observed. When working with HEPES, it is advisable to use appropriate personal protective equipment such as gloves and safety goggles. The compound is stable under normal storage conditions and does not require refrigeration. Its water solubility means it should be handled carefully to avoid spills or exposure, especially in environments where sensitive biological materials are being processed.

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