TCI H0917 Calcium 3-Hydroxy-3-methylbutyrate is the calcium salt of 3-hydroxy-3-methylbutyric acid, a compound far more widely known by its abbreviation HMB. It is a natural metabolite of the amino acid leucine and has become one of the important reference materials in research on muscle protein metabolism as well as in sports nutrition science. In the laboratory, this material is used as an analytical standard, as a test substance in cell and animal metabolism studies, and as a comparison material in the development and verification of analytical methods for nutritional supplement products.
The property that makes it practical to work with is its calcium salt form. Compared with the free acid, the salt form is considerably easier to handle as a solid, more stable in storage, and easier to weigh out accurately when preparing test solutions at precise concentrations. Its branched beta-hydroxy acid structure gives it a distinctive chemical character that is at the same time readily recognisable in chromatographic analysis and in mass spectrometry. Because of its status as a well-studied leucine metabolite, the compound serves as an established point of reference when researchers compare results between laboratories or between methods.
In Indonesian laboratories, this material is typically found in university nutrition and biochemistry research groups, in sports science laboratories, and in the quality control units of nutritional supplement manufacturers. It supports routine work such as preparing calibration standards, confirming the identity of an active ingredient in a finished formulation, and running metabolism experiments that require a reference compound of consistent, well-documented character across repeated studies.
Related TCI products
- Analytical standard preparation: the calcium salt weighs out reliably as a free-flowing solid, allowing the accurate gravimetric preparation of stock and calibration solutions for quantitative HMB determination.
- Muscle protein metabolism research: as a natural leucine metabolite, it serves directly as the test compound in cell culture and animal studies investigating protein synthesis and breakdown pathways.
- Sports nutrition science studies: the compound is a well-documented reference material in this field, giving researchers an established basis for comparing their findings against the published literature.
- Analytical method development and verification: its distinctive branched beta-hydroxy acid structure is readily recognised by chromatographic and mass spectrometric detection, making it suitable for developing and validating new assay methods.
- Supplement product quality control: it acts as a comparison material for confirming the identity and content of HMB declared in nutritional supplement formulations during routine product verification work.
| Brand | TCI (Tokyo Chemical Industry) |
|---|---|
| CAS number | 135236-72-5 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Building Blocks > Non-Heterocyclic Building Blocks |
| Pack sizes | available in standard TCI research packaging; please confirm the currently listed pack size options when ordering |
| Physical form | solid |
| Storage | keep in a tightly closed container under the conditions stated on the manufacturer's label |
- Chemical form: calcium salt of 3-hydroxy-3-methylbutyric acid (HMB)
Store the container tightly closed in a cool, dry place, away from moisture, direct sunlight, and sources of heat, following the storage conditions printed on the manufacturer's label. Keep the material in its original container or in a clean, well-sealed glass or chemically compatible plastic vessel, clearly labelled with the substance name and CAS number. Handle the solid in a well-ventilated area or fume hood to avoid raising and inhaling dust, and wear standard laboratory personal protective equipment including a lab coat, safety glasses, and suitable gloves. Use a clean, dry spatula for each transfer to prevent cross-contamination and moisture uptake, and reseal the container promptly after weighing. Wash hands thoroughly after handling, and consult the manufacturer's safety data sheet before first use.
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