5-Hydroxyuridine TCI H1865

TCI H1865 is 5-Hydroxyuridine, a pyrimidine nucleoside carrying a hydroxyl group at position five of the uracil ring and attached to a ribose sugar. The compound belongs to the family of modified nucleosides that have become important subjects of study in nucleic acid chemistry, particularly when researchers trace how oxidative damage or naturally occurring modifications affect the structure and function of RNA. In everyday laboratory practice, a pure material of this kind serves as an identification standard, a synthetic starting material, and a comparison reference that confirms a signal detected in a biological sample truly originates from the intended chemical species.

The characteristic that makes this nucleoside a preferred choice is the phenolic hydroxyl group on the uracil ring, which alters the electronic character of the base and at the same time opens distinctive reaction pathways, for example selective derivatisation or the formation of additional hydrogen bonds that ordinary uridine cannot provide. The molecule is highly polar because it is rich in hydroxyl groups, so it dissolves readily in water or polar solvents when stock solutions are prepared. This same property makes the compound well suited to analysis by reversed-phase chromatography combined with UV detection or mass spectrometry, two techniques that form the backbone of modern nucleoside work.

In Indonesian laboratories, materials of this type are typically used in university research groups, biochemistry and molecular biology teaching laboratories, and analytical service units that support nucleic acid studies. They are commonly employed when a reference substance is required to validate a chromatographic method, when a modified nucleoside is needed as a building block for synthesis, or when a group is establishing an analytical procedure and must demonstrate that its identification of a target species is chemically sound rather than inferred.

• Analytical reference standard for nucleoside profiling, providing a defined pure species that allows retention time and spectral response to be matched confidently against peaks observed in complex biological digests.
• Method development for reversed-phase HPLC, where the compound's high polarity and UV-absorbing uracil ring make it a convenient probe for optimising gradient conditions, column selection, and detector settings.
• Mass spectrometry calibration and confirmation work, supplying a known modified nucleoside whose fragmentation behaviour helps researchers assign signals correctly when screening RNA hydrolysates for oxidative modifications.
• Synthetic starting material in nucleic acid chemistry, since the phenolic hydroxyl at position five offers a selective handle for derivatisation routes that are not accessible from unmodified uridine.
• Research into oxidative damage and natural RNA modification, functioning as an authentic comparison compound when investigators examine how such changes influence base pairing, structure, and downstream biological function.

• Product Code: H1865

Store the container in a cool, dry place, tightly closed and protected from light and moisture, following the storage conditions printed on the manufacturer's label. Original supplier packaging is the most suitable container; if the material must be transferred, use clean, chemically compatible glass vials with secure closures and label them clearly with the compound name, CAS number, and date of transfer. Handle the material in a well-ventilated area or fume hood using standard laboratory personal protective equipment, including a laboratory coat, safety glasses, and suitable gloves. Avoid generating dust when weighing, keep the container closed when not in use, and consult the manufacturer's safety data sheet before first handling and before disposal.

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