2-Morpholinoethanesulfonic Acid (MES) is a chemical compound widely used as a buffer in biochemical and molecular biology experiments. It plays a crucial role in maintaining a stable pH environment, which is essential for the accuracy and reproducibility of laboratory procedures. MES is particularly valuable in applications where precise pH control is required, such as in enzymatic reactions, cell culture, and electrophoresis. Its ability to resist changes in pH under various experimental conditions makes it a reliable component in many research protocols.
MES is preferred due to its chemical stability and resistance to degradation, ensuring consistent performance across multiple uses. It is non-toxic and has a neutral odor, making it safe for use in diverse laboratory settings. Additionally, its solubility and melting point characteristics allow for easy handling and processing. These properties contribute to its widespread adoption in both academic and industrial research environments.
In Indonesian laboratories, MES is commonly used in research and educational institutions, particularly in the fields of biology, chemistry, and medicine. Its availability and cost-effectiveness make it a popular choice among researchers and students. MES is frequently employed in PCR reaction solutions and other pH-sensitive applications, supporting a wide range of experimental needs.
- Molecular Biology: MES is ideal for maintaining pH stability during PCR reactions, ensuring optimal enzyme activity and DNA amplification.
- Biochemical Analysis: It provides a controlled environment for enzymatic reactions, enhancing the accuracy of biochemical assays.
- Electrophoresis: MES helps maintain a consistent pH during gel electrophoresis, ensuring reliable separation of nucleic acids and proteins.
- Cell Culture: It supports the growth of cells in culture media by maintaining a stable pH, which is critical for cell viability.
- Buffer Solutions: MES is used to formulate custom buffer solutions for various experimental setups requiring precise pH control.
| Brand | TCI |
|---|---|
| CAS number | 4432-31-9 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Biological Buffers |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid powder |
| Storage | Store in a cool, dry place away from moisture and direct sunlight |
MES should be stored in a cool, dry place, away from moisture and direct sunlight to maintain its chemical integrity. It is recommended to use airtight containers to prevent exposure to humidity, which could affect its performance. Due to its non-toxic nature, it is generally safe to handle, but standard laboratory precautions should still be followed. Avoid contact with skin and eyes, and ensure proper ventilation when working with large quantities. MES does not require special handling beyond standard chemical safety practices, making it suitable for routine laboratory use.
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