2-Maleimidoacetic Acid TCI M3143

TCI M3143 2-Maleimidoacetic Acid is a bifunctional linking reagent that combines a maleimide group and a carboxylic acid group within a single small molecule. In chemical biology laboratories, this compound serves as a bridge between two molecules of differing chemical character: the maleimide group reacts selectively with free thiols such as cysteine residues on proteins or peptides, while the carboxylic acid group can be activated to form amide bonds with amines. This combination makes it a fundamental tool for assembling protein conjugates, fluorescent labels, and a wide range of other bioconjugate constructs in a controlled manner.

The property that makes this reagent a preferred choice is the highly selective reactivity of maleimide toward thiols across the slightly acidic to neutral pH range, allowing the reaction to proceed without disturbing amine groups on the protein. The very short linker distance is an advantage in its own right when researchers wish to minimise flexibility and spacing between the two components of a conjugate, for example so that molecular orientation is preserved. The carboxylic acid group provides flexibility of activation through carbodiimide coupling reagents or active ester formation, so that the conjugation sequence can be planned according to the needs of each experiment.

In Indonesian laboratories, this reagent is typically used in university chemical biology and biochemistry research groups, biotechnology laboratories, and analytical development units working on protein labelling and bioconjugation. It is generally purchased in small quantities appropriate to synthetic-scale and preparative-scale work, then stored under controlled conditions and used according to a defined coupling protocol. Its role is that of a supporting building block within a broader conjugation workflow rather than a stand-alone end product.

• Protein and peptide bioconjugation: the maleimide group reacts selectively with free cysteine thiols, allowing a defined attachment point on the biomolecule without modifying lysine amine residues elsewhere on the structure.
• Fluorescent label attachment: the carboxylic acid terminus can be activated and coupled to an amine-bearing dye, while the maleimide end anchors the resulting construct to a thiol-containing protein or peptide.
• Short-spacer crosslinking studies: the very small linker distance minimises flexibility between the two joined components, which is valuable when the relative orientation of the conjugate partners must be preserved.
• Heterobifunctional linker chemistry development: the combination of thiol-reactive and amine-reactive functionality in one molecule allows two-step, sequential conjugation strategies to be designed with controlled reaction order.
• Carbodiimide and active ester coupling work: the free carboxylic acid is compatible with standard carbodiimide coupling reagents or conversion to an active ester, supporting routine amide bond formation with amine partners.

• Product Code: M3143

Store this reagent in its original tightly closed container in a cool, dry place away from direct sunlight, moisture, and sources of heat or ignition, following the storage conditions stated on the manufacturer's label. Maleimide reagents are moisture sensitive, so the container should be allowed to reach room temperature before opening to avoid condensation, and resealed promptly after use. Handle the material in a fume hood using appropriate personal protective equipment, including safety goggles, gloves, and a laboratory coat. Avoid contact with skin and eyes and avoid inhaling any dust. Keep the reagent separate from incompatible materials, ensure good ventilation in the storage area, and consult the manufacturer's safety data sheet before use and before disposing of any waste.

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