4-Nitrophenol (0.25% in Water) [for pH Determination] TCI N0161

Chemical Identity

CAS No.: 100-02-7
PubChem: CID 980·SID 87573544
Formula: C6H5NO3
Molecular weight: 139.11 g/mol
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 4-nitrophenol
SMILES: C1=CC(=CC=C1[N+](=O)[O-])O
InChIKey: BTJIUGUIPKRLHP-UHFFFAOYSA-N

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Volume 500mL

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4-Nitrophenol (0.25% in Water) is a specialized chemical reagent used in laboratories for pH determination. It serves as an indicator in pH measurement processes, responding to changes in the acidity or alkalinity of a solution. This reagent is particularly useful in analytical chemistry, where accurate and reliable pH readings are essential for various experimental procedures. Its formulation in water makes it convenient for use without the need for additional solvents, simplifying the preparation process for laboratory technicians and researchers.

The properties of 4-Nitrophenol make it a preferred choice for pH testing. It is water-soluble, which ensures uniform dispersion in solution, and its yellow-brown color provides a clear visual response to pH changes. The 0.25% concentration ensures stability during testing, reducing the risk of interference with measurement results. Additionally, it does not contain hazardous chemicals that could damage laboratory equipment or pose safety risks to users. These characteristics contribute to its reliability and ease of use in various analytical applications.

In Indonesian laboratories, 4-Nitrophenol is widely used across multiple disciplines, including analytical chemistry, environmental science, and industrial research. Its simplicity and effectiveness make it a go-to reagent for routine pH testing. It is commonly found in research institutions, quality control labs, and educational facilities, supporting both academic and industrial applications with consistent performance and accuracy.

TCI brochures (PDF)

Titration Reagents and TLC Stains 2026-05-07 · p. 3

Related TCI products

pH Testing in Environmental Analysis – This reagent is ideal for assessing the acidity or alkalinity of water samples, soil extracts, and other environmental media, providing quick and reliable results for ecological studies.
Acid-Base Titration – Its color change response to pH variations makes it suitable for titration experiments, helping to identify the endpoint of neutralization reactions with precision.
Educational Laboratory Work – It is commonly used in teaching labs to demonstrate pH measurement techniques, offering students a practical and visual way to understand chemical equilibrium.
Industrial Quality Control – In manufacturing settings, it supports the monitoring of pH levels in chemical processes, ensuring product consistency and compliance with industry standards.
Research and Development – Its stability and accuracy make it a valuable tool for researchers conducting experiments that require precise pH control and measurement.

Brand	TCI
CAS number	100-02-7
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > pH Measurement Reagents
Pack sizes	100 mL, 500 mL
Physical form	Liquid solution
Storage	Store in a cool, dark place away from direct sunlight

This reagent should be stored in a cool, dark place to maintain its stability and effectiveness. It is recommended to use airtight containers to prevent contamination and evaporation. As it is a water-based solution, it should be kept away from heat sources and direct sunlight to avoid degradation. Laboratory personnel should handle it with care, using appropriate personal protective equipment when necessary. Due to its chemical nature, it should not be stored near incompatible substances. Regular monitoring of storage conditions ensures the reagent remains suitable for accurate pH testing.

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