5-Nitrofluorescein (isomer I) TCI N0199

Chemical Identity

CAS No.: 3326-35-0
PubChem: CID 76846·SID 87573570
Formula: C20H11NO7
Molecular weight: 377.3 g/mol
Purity: >98.0%(T)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one
SMILES: C1=CC2=C(C=C1[N+](=O)[O-])C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChIKey: UACQOMKZFGNRBL-UHFFFAOYSA-N

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5-Nitrofluorescein (isomer I) is a chemical compound widely used as a fluorescent dye in laboratory experiments. It belongs to the class of fluorescent dyes, which are capable of absorbing light and emitting it at specific wavelengths. This property makes it a valuable tool for identifying and analyzing other compounds through fluorescence techniques. In research settings, it is commonly used for detecting and quantifying substances in analytical and biochemical studies. Its ability to emit light under specific conditions allows for precise observation and measurement, making it an essential component in many scientific investigations.

The compound is known for its stability under controlled conditions and its solubility in organic solvents, which enhances its usability in various chemical reactions. Its fluorescent response is clear and consistent, enabling researchers to monitor changes or reactions effectively. The chemical’s sensitivity to extreme environments also means that it requires careful handling to maintain its integrity. These characteristics make it a preferred choice for applications that demand accuracy and reliability in fluorescence-based experiments.

In Indonesian laboratories, 5-Nitrofluorescein is extensively used in chemical and biological research. Educational institutions and research organizations frequently employ it in experiments related to fluorescence spectroscopy and analytical chemistry. Its availability through suppliers like AMI Scientific ensures that it remains a key reagent in both academic and industrial research settings.

Related TCI products

Fluorescence Spectroscopy: This item is ideal for fluorescence spectroscopy due to its ability to emit light at specific wavelengths, allowing precise measurement of molecular interactions and concentrations.
Biochemical Analysis: It is well-suited for biochemical analysis as it provides clear fluorescent signals, aiding in the detection of biomolecules and metabolic processes.
Environmental Monitoring: Its sensitivity to chemical changes makes it useful in environmental monitoring for detecting pollutants and assessing water quality.
Drug Development: It supports drug development by enabling the visualization of molecular interactions and the evaluation of drug efficacy in experimental models.
Toxicology Studies: The compound is used in toxicology studies to track the distribution and metabolism of substances in biological systems through fluorescence imaging.

Brand	TCI
CAS number	3326-35-0
Molecular formula	—
Purity	—
Category	Chemistry > Stains and Dyes (for Research and Experimental Use) > Stains and Dyes (Other) (for Research and Experimental Use)
Pack sizes	Available in various pack sizes as per supplier specifications
Physical form	Solid powder
Storage	Store in a cool, dry place away from direct light

5-Nitrofluorescein should be stored in a cool, dry place, away from direct light and sources of heat. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its sensitivity to extreme chemical environments, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. It is important to ensure that the compound is not exposed to strong acids or bases, as this may affect its fluorescent properties. Proper labeling of containers and adherence to standard laboratory safety protocols are essential to maintain the integrity and effectiveness of the compound in research applications.

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