Propidium Iodide [for Biochemical Research] TCI P3234

Chemical Identity

CAS No.: 25535-16-4
Purity: >90.0%(HPLC)(qN

Rp 1.220.100,00

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Propidium Iodide (PI) is a fluorescent stain widely used in biochemical research and molecular biology. It serves as a vital tool for identifying damaged or dead cells by selectively entering cells with compromised cell membranes. Once inside, it binds to DNA and emits a strong fluorescent signal detectable under a fluorescence microscope. This property makes it indispensable for cell viability assays and apoptosis studies. PI is particularly useful in analyzing cellular health and detecting changes in cell structure, making it a key reagent in many laboratory workflows.

One of the key reasons PI is preferred is its strong fluorescence and high sensitivity to DNA. It is non-toxic to living cells, ensuring it does not interfere with biological processes during experiments. Additionally, PI exhibits good stability under certain conditions, allowing for repeated use in various experimental setups. These characteristics make it a reliable and consistent choice for researchers working in both academic and clinical settings. Its ability to provide clear and reproducible results further enhances its utility in scientific investigations.

In Indonesian laboratories, Propidium Iodide is commonly used in molecular biology, pharmacology, and medical research. It supports a wide range of applications, from basic research to diagnostic testing. Institutions, universities, and clinical laboratories rely on PI to accelerate cellular analysis and improve the accuracy of their findings. Its relevance in both academic and applied research underscores its importance in the Indonesian scientific community.

TCI brochures (PDF)

Organelle Research Reagents 2026-08-06 · p. 2
Quaternary Ammonium Salts 2025-11-10 · p. 7

Related TCI products

Cell viability assays where PI is used to distinguish between live and dead cells due to its ability to enter only compromised cell membranes.
Apoptosis studies as PI can detect DNA fragmentation and membrane permeabilization associated with programmed cell death.
Fluorescence microscopy applications where PI's strong fluorescence allows for clear visualization of cellular structures and DNA content.
Tumor cell analysis in cancer research, where PI helps identify and quantify dead or dying cancer cells during treatment evaluation.
Diagnostic testing in clinical laboratories where PI aids in assessing cell health and detecting pathological changes in biological samples.

From the TCI brochure

Biochemical research

Brand	TCI
CAS number	25535-16-4
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	As per supplier specifications
Physical form	Solid (powder or crystalline)
Storage	Store at 2–8°C in a dark, dry place away from light and moisture

Propidium Iodide should be stored at a temperature between 2–8°C to maintain its stability and effectiveness. It is recommended to keep the product in a dark, airtight container to prevent degradation caused by light exposure. Due to its fluorescent properties, it is important to handle PI with care to avoid contamination. While it is non-toxic to living cells, it should still be treated as a chemical reagent with appropriate laboratory safety measures. Always ensure that the container is properly sealed and stored in a secure location away from incompatible substances. Proper handling and storage conditions help preserve the integrity of the reagent for accurate and reliable experimental results.

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