TCI T0040 Tetrabromophenol Blue Disodium Salt is a sulfonphthalein dye supplied as the disodium salt. Laboratories use it as an acid-base indicator and as a research dye. Its molecule carries several bromine atoms on a phenol framework, and these atoms shape how its colour responds to changes in acidity. Researchers use it to follow pH changes by eye, to prepare indicator solutions, and to develop colour-based detection methods in research and experimental work.
Tetrabromophenol blue is widely known as a "protein error" indicator. Its colour shifts when it interacts with proteins at a fixed pH, and many semi-quantitative protein tests rely on this principle. As a disodium salt, it usually dissolves in aqueous solvents faster than its free acid form. Stock solutions can therefore be prepared with little or no organic solvent, which makes working solutions more practical to make. The colour change from yellowish to a distinct blue is clear and easy to read. That makes it suitable both for visual observation and for simple spectrophotometric measurements.
In Indonesian laboratories, this dye is relevant to university chemistry and biochemistry departments, research institutes, and analytical laboratories that run indicator-based or colorimetric procedures. Teaching laboratories can use it to demonstrate acid-base indicator behaviour and protein-dye interactions. Research groups can include it when developing or checking colour-based detection methods. Because it dissolves readily in water, it suits laboratories that prefer aqueous preparations and want to limit their use of organic solvents in routine work.
TCI brochures (PDF)
- Titration Reagents and TLC Stains 2026-05-07 · p. 2
- Acid-base indication: the dye changes colour clearly with acidity, so analysts can follow pH changes by eye during titrations and routine checks.
- Protein-error testing: its colour shifts when it interacts with proteins at a fixed pH, which is the principle behind many semi-quantitative protein tests.
- Indicator solution preparation: the disodium salt dissolves readily in aqueous solvents, so stock and working indicator solutions can be made with little organic solvent.
- Colorimetric method development: the clear change from yellowish to blue gives researchers an easy-to-read signal when designing new colour-based detection methods.
- Simple spectrophotometric measurement: the distinct colour forms of the dye can be measured with basic spectrophotometers, alongside visual observation, for semi-quantitative laboratory analysis.
| Brand | TCI |
|---|---|
| CAS number | not stated in this listing; please check the TCI product label or certificate of analysis |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Stains and Dyes (for Research and Experimental Use) > Stains and Dyes (Other) (for Research and Experimental Use) |
| Pack sizes | available in TCI standard packaging; please confirm details with our sales team or on the product label |
| Physical form | — |
| Storage | follow the storage conditions on the TCI label and Safety Data Sheet |
- Product code: T0040
Store Tetrabromophenol Blue Disodium Salt in its original, tightly closed container in a cool, dry place away from direct sunlight, moisture, and incompatible chemicals. Follow the storage temperature shown on the TCI label. Once you have prepared indicator solutions, keep them in clean, clearly labelled glass or chemically compatible plastic bottles, and record the preparation date. When handling the solid or its solutions, wear standard laboratory protective equipment such as gloves, safety glasses, and a lab coat. Avoid creating or breathing in dust, and avoid contact with skin and eyes. Read the Safety Data Sheet before use, and dispose of waste according to institutional and local regulations. This product is for research and experimental use only.
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