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TCI

CAS 50-89-5

Thymidine TCI T0233

Thymidine TCI T0233

Chemical Identity

CAS No.
50-89-5
PubChem
CID 5789·SID 87576219
Formula
C10H14N2O5
Molecular weight
242.23 g/mol
Purity
>98.0%(HPLC)(T)
Reference databases
ChEBI·ChemSpider·ECHA·Wikipedia
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
SMILES
CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
InChIKey
IQFYYKKMVGJFEH-XLPZGREQSA-N
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Thymidine is a chemical compound classified as a nucleotide and is a key component of DNA. It plays a fundamental role in molecular biology and biochemistry, serving as a building block for DNA synthesis and various genetic research applications. In laboratory settings, thymidine is essential for processes such as polymerase chain reaction (PCR), cloning, and genetic analysis. Its presence is critical in experiments that require the manipulation and study of nucleic acids, making it a vital reagent for researchers working in molecular biology and genetics.

Thymidine is valued for its chemical stability under controlled conditions, although it is sensitive to light and high temperatures. It is typically available in crystalline or solid form and dissolves well in organic solvents like ethanol and water. These properties make it a preferred choice for biochemical experiments where purity and solubility are important factors. Its non-toxic nature also contributes to its widespread use, as it allows for safer handling in laboratory environments.

In Indonesian laboratories, thymidine is commonly used in molecular biology, pharmacology, and medical research. It supports the development of new drugs, genetic analysis, and studies on genetic disorders. Its role in DNA synthesis and enzyme activity testing makes it an indispensable tool for researchers aiming to advance scientific knowledge in these fields.

  • DNA synthesis and polymerase chain reaction (PCR) experiments benefit from thymidine as it is a fundamental nucleotide required for DNA strand elongation.
  • Genetic analysis and sequencing processes rely on thymidine to ensure accurate DNA template creation for sequencing technologies.
  • Enzyme activity assays use thymidine to evaluate the function of DNA polymerases and other nucleotide-related enzymes.
  • Cloning and gene expression studies require thymidine to support the replication and transcription of genetic material.
  • Research into genetic disorders and mutations often utilizes thymidine to study how nucleotide modifications affect DNA structure and function.

Brand TCI
CAS number 50-89-5
Molecular formula —
Purity —
Category Life Science > Biochemicals and Reagents > Nucleosides, Nucleotides, Oligonucleotides
Pack sizes Available in various quantities as per standard laboratory reagent packaging
Physical form Solid or crystalline
Storage Store in a cool, dark place away from light and high temperatures

Thymidine should be stored in a cool, dark environment to prevent degradation due to light exposure. It is recommended to keep it in airtight containers to minimize moisture absorption and contamination. Due to its sensitivity to high temperatures, it should be kept away from heat sources and direct sunlight. Proper labeling of containers is essential to ensure safe handling and prevent mix-ups in the laboratory. Regular checks for contamination and purity should be conducted to maintain the integrity of the reagent. All laboratory personnel should follow standard safety protocols when handling thymidine to ensure a safe and effective working environment.

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