4-Bromophenacyl Bromide [for HPLC Labeling] TCI A5501

Chemical Identity

CAS No.: 99-73-0
PubChem: CID 7454·SID 87563151
Formula: C8H6Br2O
Molecular weight: 277.94 g/mol
Purity: >99.0%(T)(GC)
Reference databases: ChemSpider·ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2-bromo-1-(4-bromophenyl)ethanone
SMILES: C1=CC(=CC=C1C(=O)CBr)Br
InChIKey: FKJSFKCZZIXQIP-UHFFFAOYSA-N

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4-Bromophenacyl Bromide is a chemical compound widely used in analytical chemistry, particularly in derivatization processes for HPLC labeling. It functions as a labeling agent, aiding in the identification and detection of target compounds within liquid chromatography columns. This reagent is essential in enhancing the sensitivity and specificity of analytical methods, especially for compounds that are less responsive to standard detection techniques. Its role in modern laboratory workflows is critical for achieving accurate and reliable results in complex sample analyses.

The compound is chemically stable and exhibits good reactivity, making it a preferred choice for high-precision laboratory environments. Its molecular structure enables it to react effectively with organic compounds, particularly those containing hydroxyl or amine groups. These chemical properties make it highly versatile and suitable for a variety of analytical applications. Its reliability and consistency ensure that it meets the rigorous demands of both academic and industrial research settings.

In Indonesian laboratories, 4-Bromophenacyl Bromide is commonly used in scientific research and chemical quality testing. It is a valuable resource for higher education institutions, research laboratories, and companies requiring accurate and dependable chemical analysis. Its application supports a wide range of analytical needs, contributing to the advancement of scientific studies and quality control processes.

Advantages (TCI brochure)

Bromoacetyl group reacts easily with a carboxy group to form the ester in the presence of a base
Resulting ester is stable and reaches the detector without decomposition in reversed phase HPLC
Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

Related TCI products

HPLC Labeling: This reagent is ideal for labeling compounds in HPLC to enhance detection and identification, particularly for substances that are difficult to detect using standard methods.
Derivatization Reactions: Its reactivity with hydroxyl and amine groups makes it suitable for modifying target compounds to improve their detectability in chromatographic analysis.
Analytical Chemistry Research: It is frequently used in research settings to develop and validate analytical methods for complex sample matrices.
Quality Control Testing: Its stability and reactivity make it a reliable tool for testing the purity and composition of chemical samples in industrial and academic laboratories.
Compound Identification: It aids in the identification of unknown compounds by facilitating their detection through enhanced chromatographic responses.

From the TCI brochure

Fatty acids (as 4-bromophenacyl esters)
Dicarboxylic acids
Synthetic prostaglandins
Unsaturated fatty acids
Alkyl methylphosphonate
Ganglioside
Betaine

Brand	TCI
CAS number	99-73-0
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in various sizes as per standard reagent packaging
Physical form	Solid or liquid, depending on the specific product formulation
Storage	Store in a cool, dry place away from direct sunlight and moisture

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC C1-60-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 80/20; 1 mL/min; asam lemak sebagai 4-bromofenasil ester)

Source: TCI brochure A1096 (2026-03-10)

This reagent should be stored in a cool, dry environment to maintain its chemical stability and reactivity. It is recommended to keep it in a tightly sealed container to prevent exposure to moisture and air. Proper ventilation is essential when handling the compound to minimize inhalation risks. Avoid contact with skin and eyes, and use appropriate personal protective equipment such as gloves and safety goggles. Ensure that storage areas are free from incompatible materials and that the compound is kept out of reach of children and unauthorized personnel. Regular monitoring of storage conditions will help ensure the reagent remains effective and safe for use in laboratory applications.

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