N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea [for HPLC Labeling] TCI A5506

Chemical Identity

CAS No.: 2978-11-2
PubChem: CID 76317·SID 87563156
Formula: C14H21N3O3
Molecular weight: 279.33 g/mol
Purity: >95.0%(HPLC)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (4-nitrophenyl)methyl N,N'-di(propan-2-yl)carbamimidate
SMILES: CC(C)NC(=NC(C)C)OCC1=CC=C(C=C1)[N+](=O)[O-]
InChIKey: FYDMCLCYYCIDMZ-UHFFFAOYSA-N

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N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea is a chemical compound widely used in analytical chemistry for derivatization processes in high-performance liquid chromatography (HPLC). This reagent plays a crucial role in laboratory settings by enabling the detection and identification of compounds through labeling techniques. Its application is essential in enhancing the sensitivity and accuracy of analytical results, particularly in complex sample matrices. The compound is specifically designed for use in HPLC labeling, where it helps improve the resolution and clarity of chromatographic peaks.

The compound’s chemical properties make it a preferred choice for analytical applications. It exhibits stability and reactivity, allowing it to form effective complexes with target compounds. This reactivity ensures that the derivatization process is both efficient and reliable, contributing to the overall accuracy of analytical data. Its molecular structure is tailored to interact specifically with analytes, making it a versatile tool in various analytical workflows. The compound’s ability to enhance detection limits and improve chromatographic performance is a key factor in its widespread use.

In Indonesian laboratories, this reagent is commonly used in research and testing environments. It is particularly favored in fields such as pharmaceuticals, environmental science, and food analysis. Its role in improving the precision and reliability of analytical results makes it an essential component in many laboratory protocols. The compound’s consistent performance and compatibility with standard analytical techniques ensure its continued relevance in both academic and industrial settings.

Advantages (TCI brochure)

Reacts with a carboxy group to form the ester without a catalyst or activating agent
Resulting ester is stable and reaches the detector without decomposition in reversed phase HPLC
Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

Related TCI products

Used in pharmaceutical research for the identification and quantification of complex drug compounds due to its ability to enhance detection sensitivity.
Applied in environmental analysis to detect trace organic pollutants in water and soil samples, improving the accuracy of chromatographic results.
Employed in food safety testing to identify and analyze contaminants in food products, ensuring compliance with regulatory standards.
Utilized in clinical laboratories for the analysis of biomolecules, providing reliable data for diagnostic and research purposes.
Integrated into routine analytical workflows for the derivatization of amino acids and peptides, supporting high-resolution HPLC analysis.

From the TCI brochure

Fatty acids (as 4-nitrobenzyl esters)

Brand	TCI
CAS number	2978-11-2
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in standard laboratory quantities
Physical form	Solid or liquid, depending on formulation
Storage	Store in a cool, dry place away from direct light

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC C1-60-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 75/25; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

This reagent should be stored in a cool, dry environment, away from direct sunlight and sources of heat. It is recommended to use airtight containers to prevent moisture absorption and maintain chemical integrity. Proper labeling of storage containers is essential to ensure safe handling and prevent cross-contamination. Laboratory personnel should wear appropriate personal protective equipment, such as gloves and safety goggles, when handling the compound. The reagent should be kept in a well-ventilated area to minimize exposure risks. Regular inspection of storage conditions is advised to ensure the reagent remains in optimal condition for analytical use.

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