N-Chloromethyl-4-nitrophthalimide [for HPLC Labeling] TCI A5503

Chemical Identity

CAS No.: 54455-37-7
PubChem: CID 3016883·SID 87563153
Formula: C9H5ClN2O4
Molecular weight: 240.60 g/mol
Purity: >98.0%(T)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2-(chloromethyl)-5-nitroisoindole-1,3-dione
SMILES: C1=CC2=C(C=C1[N+](=O)[O-])C(=O)N(C2=O)CCl
InChIKey: YXMUGXUCSVUONH-UHFFFAOYSA-N

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N-Chloromethyl-4-nitrophthalimide is a chemical compound widely used in analytical chemistry for derivatization processes. It plays a crucial role in laboratories where high-performance liquid chromatography (HPLC) labeling is required. This reagent is specifically designed to enhance the detectability of target compounds during analytical procedures. Its application is essential in ensuring accurate identification and quantification of substances in complex mixtures. By forming stable complexes with organic compounds, it simplifies the analysis process and improves the sensitivity of detection methods.

The compound is valued for its reactivity and stability, making it a preferred choice in analytical applications. Its ability to selectively react with specific organic molecules allows for targeted derivatization, which is critical in chromatographic analysis. The chemical structure of N-Chloromethyl-4-nitrophthalimide ensures that it can be used in a wide range of analytical techniques without interfering with the results. Its reliability and consistency in performance contribute to its popularity among researchers and analysts.

In Indonesian laboratories, N-Chloromethyl-4-nitrophthalimide is commonly used in scientific research and industrial applications that require precise chemical analysis. It is a key component in various analytical workflows, supporting both academic and commercial research. Its availability and quality make it a trusted reagent for institutions and companies that prioritize accuracy and reliability in their analytical processes.

Advantages (TCI brochure)

Chloromethyl group reacts with a carboxy group to form the ester in the presence of a base
Resulting ester is stable and reaches the detector without decomposition in reversed phase HPLC
Excellent chromatogram obtained by measuring at 230 nm

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

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From the TCI brochure

Fatty acids (as (4-nitrophthalimido)methyl esters)

Brand	TCI
CAS number	54455-37-7
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in standard laboratory quantities as per supplier specifications
Physical form	Solid powder
Storage	Store in a cool, dry place away from moisture and direct sunlight

Data from the TCI brochure

Panjang gelombang deteksi UV	230 nm (Kolom Kaseisorb LC ODS-300-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 85/15; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

N-Chloromethyl-4-nitrophthalimide should be stored in a cool, dry environment to maintain its chemical integrity and stability. It is recommended to keep the compound in a tightly sealed container to prevent exposure to moisture and air. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. Avoid direct contact with skin and inhalation of vapors. It is best stored in a well-ventilated area, away from incompatible materials. Proper storage ensures the reagent remains effective for its intended analytical applications.

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