DBD-F, also known as 4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, is a fluorescent compound widely used in laboratory settings for HPLC labeling. This compound serves as a fluorescent tag to identify and track specific molecules during high-performance liquid chromatography procedures. Its role is critical in analytical chemistry and biochemistry, where precise molecular identification is essential. DBD-F is particularly valuable for its ability to provide clear and consistent fluorescence signals, making it an indispensable tool in modern research environments.
DBD-F is preferred due to its strong fluorescence properties and excellent chemical stability. These characteristics ensure that it maintains its integrity under various experimental conditions, providing reliable and reproducible results. The compound’s resistance to degradation allows for longer retention of its fluorescent properties, which is crucial for accurate data collection over extended periods. Additionally, its compatibility with standard HPLC systems makes it a versatile choice for a wide range of applications.
In Indonesian laboratories, DBD-F is commonly used in biochemical, pharmacological, and environmental research. It supports molecular analysis techniques and is integral to studies that require precise labeling and detection. Its availability enhances the capacity of research institutions to conduct advanced analytical work, contributing to the advancement of scientific knowledge in various fields.
Advantages (TCI brochure)
- Yellow fluorescence from DBD core
- Can be labeled to amino or thiol groups
TCI brochures (PDF)
- Fluorescent Labeling Dyes 2025-11-18 · p. 2
- HPLC Labeling Reagents 2026-03-10 · p. 3
- Reagents for Biological Research 2025-03-11 · p. 4
Related TCI products
- TCI A5593 29270-56-2 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
- TCI A5592 10199-89-0 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
- TCI S0503 1858255-08-9 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate
- TCI A5591 49759-20-8 NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling]
- TCI A5569 163927-32-0 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5568 163927-31-9 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- Biochemical Analysis – DBD-F is ideal for identifying and tracking molecular compounds in complex mixtures due to its strong fluorescence and stability.
- Pharmacological Research – It is used to label and monitor drug molecules in biological samples, aiding in the study of drug interactions and efficacy.
- Environmental Monitoring – DBD-F helps detect and analyze pollutants in environmental samples, offering a reliable fluorescent marker for trace compound identification.
- Molecular Labeling – Its compatibility with HPLC systems makes it a preferred choice for labeling and separating complex molecular structures.
- Fluorescent Staining – It is used in fluorescence-based assays to enhance the visibility and detection of specific biomolecules in samples.
From the TCI brochure
- Labeling of amino or thiol groups
| Brand | TCI |
|---|---|
| CAS number | 98358-90-8 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains |
| Pack sizes | Available in standard laboratory quantities |
| Physical form | Solid powder |
| Storage | Store in a cool, dry place away from light |
Data from the TCI brochure
| λex,max | ca. 380 nm |
|---|---|
| λem,max | ca. 510 nm |
Source: TCI brochure LL039 (2025-11-18)
DBD-F should be stored in a cool, dry environment, away from direct light to maintain its chemical stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture absorption, which could affect its performance. When handling, it is important to follow standard laboratory safety protocols, including the use of personal protective equipment such as gloves and safety goggles. The compound should be kept in a well-ventilated area to minimize exposure. Proper storage ensures that the material remains effective for its intended applications, supporting accurate and reliable experimental results.
—
Product Categories
- Analytical ReagentsDerivatization ReagentsHPLC Derivatization Reagents
- Analytical ReagentsSpectroscopy ReagentsFluorescence/Luminescence Spectroscopy Reagents
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]
![98358-90-8 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5595.jpg?v=1769141524&width=1445)
![1858255-08-9 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510S0503.jpg?v=1768206279&width=533)
![49759-20-8 NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5591_a318064e-4c00-4a1a-b29d-d4f5b651eb6a.jpg?v=1769179467&width=533)
![163927-31-9 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5568_362479bf-2985-458e-84d4-983894568fca.jpg?v=1772176822&width=533)
![664985-43-7 DAABD-Cl [=4-[2-(Dimethylamino)ethylaminosulfonyl]-7-chloro-2,1,3-benzoxadiazole] [for Proteome Analysis] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5596_90fe1edd-4558-44b4-b82d-7e5cd07d7fac.jpg?v=1772176844&width=533)