![29270-56-2 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling] - AMI Scientific](http://amiscientific.com/cdn/shop/files/TCI2510A5593.jpg?v=1769179466&width=1946)
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NBD-F (4-Fluoro-7-nitro-2,1,3-benzoxadiazole) is a fluorescent compound widely used in biochemical and molecular analysis applications within laboratory settings. It functions as a highly sensitive fluorescent label, enabling the detection and identification of biological molecules such as proteins, nucleic acids, and lipids. Its utility in High-Performance Liquid Chromatography (HPLC) labeling makes it an essential tool for researchers aiming to track and analyze complex mixtures with precision. This compound is particularly valuable for its ability to emit light at specific wavelengths when exposed to excitation light, which simplifies the detection process in analytical procedures.
The properties of NBD-F that make it a preferred choice include its strong fluorescence, chemical stability, and resistance to extreme chemical conditions. It maintains its fluorescent properties even under varying pH levels, making it suitable for a wide range of experimental conditions. Additionally, its high sensitivity allows for the detection of trace amounts of substances, enhancing the accuracy of analytical results. These characteristics ensure that NBD-F remains a reliable and consistent reagent in laboratory environments where precision and reliability are paramount.
In Indonesian laboratories, NBD-F is commonly used in biomedical research, pharmacology, and biotechnology studies. It is a staple in research institutions and universities, where it supports various analytical techniques. Its role in facilitating accurate and efficient molecular analysis has made it an essential component of many laboratory workflows in Indonesia.
Advantages (TCI brochure)
- Green-yellow fluorescence from NBD core
TCI brochures (PDF)
- Fluorescent Labeling Dyes 2025-11-18 · p. 2
- HPLC Labeling Reagents 2026-03-10 · p. 3
- Reagents for Biological Research 2025-03-11 · p. 4
Related TCI products
- TCI A5595 98358-90-8 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5592 10199-89-0 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
- TCI A5569 163927-32-0 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5568 163927-31-9 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5558 156153-43-4 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI S0503 1858255-08-9 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate
- NBD-F is ideal for HPLC labeling due to its strong fluorescence and compatibility with chromatographic techniques, allowing for precise detection of biomolecules in complex samples.
- It is widely used in protein analysis to label and track proteins during purification and characterization processes, enhancing the accuracy of molecular identification.
- In nucleic acid studies, NBD-F helps in the detection and quantification of DNA and RNA, supporting research in genetics and molecular biology.
- Its application in lipid analysis enables researchers to study membrane composition and dynamics, contributing to biochemical and biophysical research.
- NBD-F is also employed in drug development to monitor the behavior and metabolism of pharmaceutical compounds in biological systems.
From the TCI brochure
- Can be labeled to amino groups or thiol groups
| Brand | TCI |
|---|---|
| CAS number | 29270-56-2 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains |
| Pack sizes | Available in various quantities as per standard supplier offerings |
| Physical form | Solid (as per typical fluorescent probe formulation) |
| Storage | Store in a cool, dark place away from light and moisture |
Data from the TCI brochure
| λex,max | ca. 470 nm |
|---|---|
| λem,max | ca. 530 nm |
Source: TCI brochure LL039 (2025-11-18)
NBD-F should be stored in a cool, dark environment to maintain its fluorescent properties and chemical stability. It is recommended to use airtight containers to prevent exposure to moisture and light, which can degrade the compound. Proper labeling of storage containers is essential to ensure safe handling and prevent accidental contamination. Laboratory personnel should wear appropriate personal protective equipment when handling NBD-F to minimize exposure. The compound should be kept away from incompatible substances to avoid any potential chemical interactions. Regular monitoring of storage conditions is advised to ensure the integrity of the material remains unaffected over time.
—
Product Categories
- Analytical ReagentsDerivatization ReagentsHPLC Derivatization Reagents
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]
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