DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling] TCI A5558

Chemical Identity

Other names: DBD-COCl
CAS No.: 156153-43-4
PubChem: CID 133003·SID 87563176
Formula: C11H13ClN4O4S
Molecular weight: 332.76 g/mol
Purity: >93.0%(T)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 2-[[4-(dimethylsulfamoyl)-2,1,3-benzoxadiazol-7-yl]-methylamino]acetyl chloride
SMILES: CN(C)S(=O)(=O)C1=CC=C(C2=NON=C12)N(C)CC(=O)Cl
InChIKey: NAIIBGUHMHXMNS-UHFFFAOYSA-N

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TCI A5558 is a specialized derivatization reagent used in analytical chemistry to enhance the detection and identification of compounds in high-performance liquid chromatography (HPLC) analysis. This compound, with the CAS number 156153-43-4, is designed to react with target analytes, improving their detectability through chemical modification. It plays a crucial role in laboratories where precise and sensitive analysis is required, particularly in complex sample matrices where standard detection methods may fall short. Its unique chemical structure enables it to selectively bind to specific functional groups, making it an essential tool for analytical chemists.

The reagent’s chemical properties, including its reactive aminomethyl and chloroformyl groups, contribute to its effectiveness in derivatization processes. These groups allow for selective and specific reactions, minimizing interference from other components in the sample. Its stability and ease of handling make it a preferred choice for routine and advanced analytical procedures. The reagent is also known for its high reactivity, which ensures efficient derivatization and enhances the overall performance of HPLC analysis.

In Indonesian laboratories, TCI A5558 is widely used in chemical analysis research, particularly in pharmaceutical, environmental, and food industry applications. Its ability to improve detection sensitivity and provide clear signals on HPLC columns makes it indispensable for both qualitative and quantitative analysis. Its reliability and consistent performance make it a trusted reagent in analytical workflows that demand accuracy and precision.

Advantages (TCI brochure)

Reacts with many kinds of nucleophilic groups under mild conditions
Resulting compounds are stable and reach the detector without decomposition
Excellent chromatograms obtained by fluorescence detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 3

Related TCI products

High-Performance Liquid Chromatography (HPLC) analysis benefits from TCI A5558 as it enhances the detection of compounds by improving signal clarity and sensitivity, making it ideal for complex sample matrices.
Pharmaceutical research utilizes this reagent to analyze drug compounds and metabolites, ensuring accurate identification and quantification in drug development and quality control.
Environmental analysis relies on TCI A5558 to detect trace pollutants in water and soil samples, offering a reliable method for monitoring environmental contaminants.
Food safety testing employs this reagent to identify and quantify harmful substances in food products, supporting regulatory compliance and consumer safety.
Forensic and toxicological studies use TCI A5558 for the detection of specific compounds in biological samples, aiding in the analysis of drug use and toxic exposure.

From the TCI brochure

Alcohols (androsterone)
Alpha-oxyacids (mandelic acid)
Phenols (estrone)
Amines (benzylamine)
Aromatic amines (phenetidine)
Thiols (2-mercapto-N-(2-naphthyl)-acetamide)

Brand	TCI
CAS number	156153-43-4
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in various quantities as per standard reagent packaging
Physical form	Solid or powder, depending on formulation
Storage	Store in a cool, dry place away from direct sunlight and moisture

Data from the TCI brochure

Androsterone: ex/em, detection limit	443 nm / 546 nm, 38 fmol (60 C, 30 min)
Mandelic acid: ex/em, detection limit	442 nm / 551 nm, 125 fmol (60 C, 15 min)
Estrone: ex/em, detection limit	440 nm / 543 nm, 40 fmol (60 C, 15 min)
Benzylamine: ex/em, detection limit	445 nm / 555 nm, 89 fmol (r.t. or 60 C, 15 min)
Phenetidine: ex/em, detection limit	443 nm / 553 nm, 56 fmol (60 C, 15 min)
2-Mercapto-N-(2-naphthyl)-acetamide: ex/em, detection limit	437 nm / 544 nm, 103 fmol (r.t.)
Mobile phase	CH3CN / H2O = 50 / 50 (40 C, Kaseisorb LC ODS Super, 1 mL/min, ex 450 nm, em 560 nm)

Source: TCI brochure A1096 (2026-03-10)

TCI A5558 should be stored in a cool, dry environment to maintain its chemical stability and effectiveness. It is recommended to keep the reagent in a tightly sealed container to prevent moisture absorption and contamination. Avoid exposure to direct sunlight and high humidity, as these conditions may affect the reagent’s performance. When handling, ensure proper personal protective equipment is worn, including gloves and safety goggles, to minimize direct contact. The reagent should be stored in a well-ventilated area to ensure safe handling. Always follow standard laboratory safety protocols to maintain a secure working environment.

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