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NBD-Cl (4-Chloro-7-nitro-2,1,3-benzoxadiazole) is a fluorescent compound widely used in biochemical and laboratory analysis. It serves as a fluorescent label for detecting and analyzing biological molecules during high-performance liquid chromatography (HPLC). This compound plays a crucial role in enhancing the visibility and identification of compounds through its fluorescent properties. Its application is essential in various research fields where precise molecular detection is required.
The compound is known for its strong fluorescence and chemical stability, making it a preferred choice in laboratory settings. Its ability to emit light when exposed to specific radiation allows for accurate and sensitive detection of target molecules. The chemical resistance of NBD-Cl ensures that it remains effective under various experimental conditions, minimizing degradation and enhancing reliability. These properties make it a valuable tool for researchers seeking precision and consistency in their analyses.
In Indonesian laboratories, NBD-Cl is commonly used in biochemical, pharmacological, and molecular biology research. It supports advanced analytical techniques required for the rapid identification and characterization of compounds. Its relevance extends to research institutions, universities, and companies that rely on sophisticated methods to improve the efficiency of their analytical processes. The compound is an essential component in the toolkit of any laboratory engaged in fluorescence-based detection and analysis.
Advantages (TCI brochure)
- Green-yellow fluorescence from NBD core
TCI brochures (PDF)
- Fluorescent Labeling Dyes 2025-11-18 · p. 2
- HPLC Labeling Reagents 2026-03-10 · p. 3
- Reagents for Biological Research 2025-03-11 · p. 4
Related TCI products
- TCI A5595 98358-90-8 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5593 29270-56-2 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
- TCI A5569 163927-32-0 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5568 163927-31-9 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI A5558 156153-43-4 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
- TCI S0503 1858255-08-9 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate
- Biochemical Analysis – NBD-Cl is ideal for labeling and detecting biomolecules during chromatographic separations due to its strong fluorescent properties.
- Pharmacological Research – It enables the tracking of drug molecules in complex biological systems, supporting drug development and metabolic studies.
- Molecular Biology Studies – NBD-Cl is used for fluorescent labeling of nucleic acids and proteins, aiding in structural and functional analysis.
- Environmental Monitoring – Its stability and sensitivity make it suitable for detecting trace compounds in environmental samples.
- Quality Control in Biotechnology – It assists in verifying the purity and integrity of biological products through fluorescence-based assays.
From the TCI brochure
- Can be labeled to amino groups or thiol groups
| Brand | TCI |
|---|---|
| CAS number | 10199-89-0 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains |
| Pack sizes | Available in various quantities as per supplier specifications |
| Physical form | Solid (as per standard product description) |
| Storage | Store in a cool, dry place away from light |
Data from the TCI brochure
| λex,max | ca. 470 nm |
|---|---|
| λem,max | ca. 530 nm |
Source: TCI brochure LL039 (2025-11-18)
NBD-Cl should be stored in a cool, dry environment, away from direct light to maintain its fluorescent properties and chemical stability. It is recommended to use airtight containers to prevent moisture ingress, which could affect its performance. As a fluorescent compound, it is sensitive to light, so proper storage conditions are essential to avoid degradation. In laboratory settings, it should be handled with care to prevent contamination. Always ensure that the storage area is secure and accessible only to authorized personnel. Regular monitoring of storage conditions is advised to maintain the compound's integrity and effectiveness for analytical use.
—
Product Categories
- Analytical ReagentsDerivatization ReagentsHPLC Derivatization Reagents
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
- HPLCHPLC Derivatization ReagentsFluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]
Produk Terkait
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