NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling] TCI A5591

Chemical Identity

Other names: NAM
CAS No.: 49759-20-8
PubChem: CID 3016496·SID 87563198
Formula: C17H10N2O2
Molecular weight: 274.27 g/mol
Purity: >98.0%(HPLC)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 1-acridin-9-ylpyrrole-2,5-dione
SMILES: C1=CC=C2C(=C1)C(=C3C=CC=CC3=N2)N4C(=O)C=CC4=O
InChIKey: NCFIKBMPEOEIED-UHFFFAOYSA-N

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Dokumen Keamanan & Mutu

↓ Unduh SDS / MSDS (PDF resmi TCI)

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N-(9-Acridinyl)maleimide (NAM) is a chemical compound widely used in biochemical and biomedical research applications. It serves as a fluorescent labeling agent, enabling researchers to track and analyze proteins in laboratory settings. This compound is particularly valuable for its ability to specifically react with cysteine residues in proteins, making it a key tool in protein detection and modification. Its fluorescent properties allow for visual identification of target proteins, which is essential in various analytical techniques. NAM is commonly used in high-performance liquid chromatography (HPLC) and Western blotting, where its labeling capabilities enhance the accuracy of protein analysis.

The chemical properties of NAM make it a preferred choice in laboratory settings. It exhibits high reactivity and stability, allowing for selective binding to proteins without altering their structure or function. This selectivity ensures reliable and consistent results in experiments. Additionally, its strong fluorescence enables high-sensitivity detection, even at low protein concentrations. The compound’s resistance to various environmental conditions further enhances its reliability across different experimental setups. These characteristics make NAM a versatile and dependable reagent for researchers.

In Indonesian laboratories, NAM is extensively used in biomedical, biochemical, and protein technology research. It is particularly favored for its accuracy and efficiency in protein detection methods. Its availability and consistent quality make it a popular choice among scientists working in these fields. NAM supports a wide range of experimental protocols, contributing to the advancement of research in both academic and industrial settings.

Advantages (TCI brochure)

Blue fluorescence
Can be labeled to thiol groups

TCI brochures (PDF)

Fluorescent Labeling Dyes 2025-11-18 · p. 3
HPLC Labeling Reagents 2026-03-10 · p. 3

Related TCI products

Fluorescent Protein Labeling: NAM is ideal for labeling proteins with high specificity and fluorescence, enabling clear visualization in techniques like Western blotting and immunofluorescence.
High-Performance Liquid Chromatography (HPLC): Its fluorescent properties allow for the detection and quantification of proteins in complex mixtures, enhancing the sensitivity and accuracy of HPLC analysis.
Protein Modification Studies: NAM's ability to react with cysteine residues makes it a valuable tool for studying protein structure and function through chemical modification.
Biochemical Assays: The compound's stability and reactivity support its use in various biochemical assays where precise protein detection is required.
Biomedical Research: NAM is widely used in biomedical applications for tracking protein interactions and behavior in cellular environments.

From the TCI brochure

Labeling of thiol groups

Brand	TCI
CAS number	49759-20-8
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	As per manufacturer specifications
Physical form	Solid (as per standard product description)
Storage	Store in a cool, dry place away from light

Data from the TCI brochure

λex,max	365 nm
λem,max	440 nm

Source: TCI brochure LL039 (2025-11-18)

N-(9-Acridinyl)maleimide should be stored in a cool, dry place away from direct light to maintain its chemical stability and fluorescence properties. It is recommended to use airtight containers to prevent moisture absorption and contamination. Due to its reactivity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. Avoid exposure to heat or prolonged light, as these can degrade the compound. In laboratory settings, it should be stored in a designated chemical storage area that is accessible only to authorized personnel. Proper labeling and documentation of storage conditions are essential for maintaining safety and compliance with laboratory protocols.

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