O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling] TCI A5532

Chemical Identity

CAS No.: 2086-26-2
PubChem: CID 74967·SID 87563167
Formula: C7H9ClN2O3
Molecular weight: 204.61 g/mol
Purity: >99.0%(HPLC)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: O-[(4-nitrophenyl)methyl]hydroxylamine;hydrochloride
SMILES: C1=CC(=CC=C1CON)[N+](=O)[O-].Cl
InChIKey: LKCAFSOYOMFQSL-UHFFFAOYSA-N

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O-4-Nitrobenzylhydroxylamine Hydrochloride is a chemical compound widely used in analytical chemistry for derivatization processes in high-performance liquid chromatography (HPLC). This reagent plays a crucial role in laboratory settings by enabling the labeling of target compounds, which simplifies their detection and identification in complex samples. Its application is essential in enhancing the sensitivity and specificity of analytical methods, particularly in the analysis of compounds with functional groups such as amines or hydroxyls.

The compound is preferred due to its stable and reactive nature, making it suitable for a variety of analytical applications. Its ability to react with organic compounds under controlled conditions ensures reliable results in chromatographic analyses. Additionally, its solubility in organic solvents facilitates its use across different analytical techniques. These properties make it a reliable choice for researchers and analysts seeking accurate and reproducible data.

In Indonesian laboratories, O-4-Nitrobenzylhydroxylamine Hydrochloride is commonly used in chemical research, environmental analysis, and chemical testing. Its role in improving the detection limits of HPLC methods makes it an essential tool for laboratories aiming to meet high analytical standards. Its widespread use in these contexts underscores its importance in both academic and industrial research settings.

Advantages (TCI brochure)

Hydroxylamino moiety reacts easily with a carbonyl group to form the corresponding oxime
Oxime is stable enough to reach the detector in reversed phase HPLC
Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

Related TCI products

High-Performance Liquid Chromatography (HPLC) labeling for enhanced detection of target compounds in complex matrices.
Environmental analysis to identify and quantify organic pollutants in water and soil samples.
Chemical testing in industrial laboratories for quality control and compound characterization.
Research applications in analytical chemistry for derivatization of amino and hydroxyl-containing compounds.
Pharmaceutical analysis to support drug development and compound identification processes.

From the TCI brochure

Aldehydes (as 4-nitrobenzyloximes)

Brand	TCI
CAS number	2086-26-2
Molecular formula	—
Purity	—
Category	Analytical Reagents > Derivatization Reagents
Pack sizes	Available in standard laboratory quantities
Physical form	Solid or powder, depending on supplier packaging
Storage	Store in a cool, dry place away from light and moisture

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC ODS-300-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 60/40; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

This compound should be stored in a cool, dry place, away from light and moisture to maintain its stability and reactivity. It is recommended to use airtight containers to prevent exposure to humidity and air. Proper labeling of storage containers is essential for safe handling. Laboratory personnel should wear appropriate personal protective equipment, such as gloves and safety goggles, when handling this reagent. It should be kept out of reach of children and stored separately from incompatible materials. Regular inspection of storage conditions ensures the reagent remains suitable for use in analytical procedures.

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