2,4-Dinitrophenylhydrazine Hydrochloride [for HPLC Labeling] TCI A5531

Chemical Identity

CAS No.: 55907-61-4
PubChem: CID 5463312·SID 87563166
Formula: C6H7ClN4O4
Molecular weight: 234.60 g/mol
Purity: >98.0%(HPLC)(T)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2,4-dinitrophenyl)hydrazine;hydrochloride
SMILES: C1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])NN.Cl
InChIKey: PUYFAZQODQZOFK-UHFFFAOYSA-N

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2,4-Dinitrophenylhydrazine Hydrochloride is a chemical compound widely used in analytical chemistry for the identification and characterization of organic compounds, particularly carbonyl compounds such as aldehydes and ketones. This reagent plays a crucial role in laboratory settings by enabling the derivatization of target compounds, which enhances their detectability and analytical performance through techniques like HPLC. Its ability to form stable hydrazone derivatives makes it an essential tool in molecular labeling and identification procedures.

The compound is valued for its chemical stability under specific conditions and its strong reactivity towards carbonyl groups. These properties ensure reliable and consistent results in analytical processes. Its specificity and reactivity make it a preferred choice for researchers aiming to achieve accurate and reproducible data. The solid form of this reagent also contributes to its practicality, as it is easy to handle and store, making it suitable for routine laboratory use.

In Indonesian laboratories, 2,4-Dinitrophenylhydrazine Hydrochloride is extensively used in chemical research, quality testing of chemical substances, and industrial sample analysis. It is commonly found in research institutions and universities, where it supports various analytical workflows. Its reliability and effectiveness have made it a standard reagent in analytical chemistry applications across the country.

Advantages (TCI brochure)

Hydrazino group reacts easily with a carbonyl group to form the corresponding hydrazone
Hydrazone is stable enough to reach the detector in reversed phase HPLC
Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

Related TCI products

Used in chemical research for the identification of carbonyl compounds due to its ability to form hydrazone derivatives.
Applied in quality control testing for the detection of aldehydes and ketones in industrial samples.
Employed in HPLC labeling procedures to enhance the detectability of target compounds through derivatization.
Utilized in analytical chemistry for molecular labeling and identification in complex sample matrices.
Integrated into routine analytical workflows for the characterization of organic compounds in both academic and industrial settings.

From the TCI brochure

Aldehydes
Keto acids
Urine and 17-ketosteroids in blood plasma
Aliphatic carbonyl compounds
Aliphatic aldehydes

Brand	TCI
CAS number	55907-61-4
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in various quantities as per standard laboratory reagent packaging
Physical form	Solid
Storage	Store in a cool, dry place away from moisture and direct sunlight

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC ODS-60-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 70/30; 1 mL/min; aldehida sebagai 2,4-dinitrofenilhidrazon)

Source: TCI brochure A1096 (2026-03-10)

This reagent should be stored in a cool, dry place, away from moisture and direct sunlight to maintain its chemical stability. It is recommended to use airtight containers to prevent exposure to air and humidity, which may affect its reactivity. Due to its chemical nature, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. It is important to ensure that it is kept out of reach of children and not stored near incompatible materials. Regular inspection of storage conditions is advised to ensure the reagent remains in optimal condition for analytical use.

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