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Description
TCI C2235 Calcium Folinate is the calcium salt of folinic acid, a reduced form of folic acid that is biologically ready for use by cells without first requiring an enzymatic reduction step. The compound belongs to the group of pharmaceutical research reagents intended for experimental use, and it serves as a source of an active folate derivative within experimental systems. In life science laboratories, this material is used to trace one-carbon metabolic pathways, to support cell growth in defined media, and to study the interactions between folate and the enzymes involved in nucleotide synthesis.
The characteristic that makes this reagent a preferred choice is its water solubility, which is considerably better than that of free folinic acid, so stock solutions are easy to prepare for both cell culture-based experiments and enzyme assays. The calcium salt form also provides better handling stability in powder form. Because the compound is already present as a reduced folate, researchers can observe its effects directly, without the additional variable introduced by the reduction process that would otherwise take place inside the cell. This directness simplifies experimental interpretation and shortens preparation work at the bench.
The 1 g and 5 g pack sizes are well matched to a reagent that is generally consumed in small quantities per experiment, which suits the working pattern of most research groups. In Indonesian laboratories, this material fits routine life science work in university research units, biochemistry and pharmacy teaching laboratories, and institutional research facilities, where reduced folate derivatives are needed for metabolic pathway studies, cell culture media supplementation, and enzyme characterisation work carried out on an experimental scale.
Laboratory Applications
One-carbon metabolism pathway studies: the compound supplies an already reduced folate derivative, allowing researchers to follow carbon transfer steps without confounding effects from intracellular reduction.
Cell culture media supplementation: its high water solubility makes it straightforward to prepare and filter stock solutions that support cell growth in defined culture media formulations.
Nucleotide synthesis enzyme research: the reagent is used to examine how folate derivatives interact with the enzymes responsible for building nucleotides in experimental systems.
Folate-enzyme interaction assays: because it is a biologically ready folate form, it can be introduced directly into enzyme assay mixtures for interaction and activity measurements.
Pharmaceutical research at experimental scale: as a pharmaceutical research reagent, it supports small-volume investigative work where an active folate source is required per experiment.
Specifications
Brand: TCI
CAS number: 1492-18-8
Category: Life Science > Biochemicals and Reagents > Pharmaceutical Research Reagents (for Experimental Use)
Pack sizes: 1 g and 5 g
Physical form: powder (calcium salt form)
Storage: store the powder under conditions that preserve its handling stability, in a closed container away from moisture
Handling and Storage
Keep the powder in its original tightly closed container to protect it from moisture, since the calcium salt form is supplied and handled as a dry solid. Store containers in a cool, dry place away from direct light and heat sources, and return the container to storage promptly after each weighing so that the remaining material is not exposed for longer than necessary. Prepare aqueous stock solutions only in the quantity needed for the planned experiment, and handle the reagent in a clean, dedicated weighing area using standard laboratory personal protective equipment, including gloves, a laboratory coat, and eye protection. Use clean spatulas and glassware to avoid cross-contamination between reagents, label all prepared solutions clearly, and follow your institution's general procedures for handling research chemicals intended for experimental use.
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