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Description
TCI D3610 2'-Deoxy-5-methylcytidine is a modified nucleoside composed of a 5-methylcytosine base linked to a 2'-deoxyribose sugar. This compound serves as the chemical equivalent of the 5-methylcytosine unit naturally present in genomic DNA, where it functions as the most widely studied epigenetic marker. In the laboratory, this material acts as a building block for chemical synthesis, a reference compound in DNA methylation content analysis, and a model substrate in enzymatic studies that investigate the addition or removal of methyl groups on nucleic acids.
What makes this nucleoside a preferred choice is its structural fidelity to the natural unit it is designed to replicate: the deoxyribose backbone remains intact, allowing the compound to be integrated into nucleotide and oligonucleotide synthesis pathways without altering established chemical approaches. The methyl group at the 5-position of the cytosine ring provides distinctive spectroscopic and chromatographic signatures, enabling the compound to be readily distinguished from ordinary cytidine during HPLC or LC-MS analysis.
As a solid that can be weighed and dissolved in a controlled manner, this material is also practical for preparing stock solutions of precise concentration. In Indonesian laboratories, researchers working in epigenetics, molecular biology, and pharmaceutical development rely on this compound to support methylation studies, oligonucleotide synthesis projects, and enzyme characterization experiments that require a faithful chemical stand-in for naturally occurring 5-methylcytosine residues in DNA.
Laboratory Applications
DNA methylation analysis: this nucleoside serves as a reliable reference standard for quantifying 5-methylcytosine content in genomic DNA samples using chromatographic and mass spectrometric detection methods.
Oligonucleotide synthesis: the intact deoxyribose backbone allows this compound to be incorporated into solid-phase synthesis workflows for producing modified DNA sequences containing 5-methylcytosine at defined positions.
Enzyme activity assays: researchers use this nucleoside as a model substrate to study the catalytic behavior of DNA methyltransferases and demethylases involved in epigenetic regulation of gene expression.
Epigenetic research: the compound provides a chemically faithful building block for constructing DNA fragments that mimic naturally methylated sequences, supporting studies on chromatin structure and gene silencing mechanisms.
Analytical method development: the distinctive chromatographic and spectroscopic profile of this nucleoside makes it a suitable marker for validating HPLC and LC-MS protocols designed to detect methylated cytosine derivatives.
Specifications
Brand: TCI
Product Code: D3610
CAS Number: 838-07-3
Category: Chemistry > Chemical Biology > Nucleic Acid Chemistry
Physical Form: Solid
Storage: Store according to the directions on the original TCI product label
Handling and Storage
Store this nucleoside in a cool, dry environment away from direct light and sources of heat, following the storage instructions provided on the original TCI product label. Keep the material in its original container or in a tightly sealed, chemically compatible vessel to prevent moisture absorption and contamination. When handling, use standard laboratory personal protective equipment including gloves, a lab coat, and safety goggles. Avoid generating dust during weighing and transfer operations, and work in a well-ventilated area or fume hood when preparing solutions.
