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Description
TCI D4116 Doxycycline Hyclate is the hyclate salt of doxycycline, a second-generation tetracycline-class antibiotic that acts by inhibiting bacterial protein synthesis at the 30S ribosomal subunit. In the life science laboratory, this compound serves two roles of equal importance. First, it functions as a reference antibiotic and test substance in microbial susceptibility testing and general microbiology research. Second, and very widely used in modern molecular biology, it acts as the inducing molecule in tetracycline-controlled gene expression systems known as the Tet-On and Tet-Off systems.
The properties that make this material a preferred choice begin with its hyclate salt form, which is considerably more water-soluble than doxycycline free base. This improved solubility simplifies the preparation of stock solutions for both cell culture media and microbiological media. Doxycycline also has a high affinity for the tetracycline repressor protein, so induction of gene expression can be achieved at low concentrations that do not interfere with the growth of mammalian cells. Its spectrum of activity is broad, covering Gram-positive bacteria, Gram-negative bacteria, and certain intracellular organisms.
In Indonesian laboratories, this material is typically used across university research groups, biotechnology laboratories, and microbiology testing facilities. It appears in antimicrobial susceptibility work, in inducible expression experiments involving mammalian cell lines, and in microbiological media preparation. Because tetracycline compounds require careful handling, laboratories normally prepare fresh stock solutions and keep working aliquots protected during routine use.
Laboratory Applications
- Tet-On and Tet-Off inducible gene expression — its high affinity for the tetracycline repressor protein allows controlled induction at concentrations low enough to leave mammalian cell growth undisturbed.
- Antimicrobial susceptibility testing — serves as a reference tetracycline-class antibiotic and test substance for determining the sensitivity of bacterial isolates under standardized microbiological conditions.
- General microbiology research — its broad spectrum against Gram-positive, Gram-negative, and certain intracellular organisms makes it a useful tool compound in bacterial growth and inhibition studies.
- Mammalian cell culture experiments — the water-soluble hyclate salt allows straightforward preparation of aqueous stock solutions that can be added directly to cell culture media without organic solvents.
- Microbiological media preparation — the compound dissolves readily in aqueous media, allowing convenient incorporation of a defined tetracycline-class antibiotic into selective or test media formulations.
Specifications
- Brand: TCI
- CAS Number: 24390-14-5
- Category: Life Science > Biochemicals and Reagents > Antibiotics
- Product Code: D4116
- Chemical Class: Second-generation tetracycline antibiotic, hyclate salt form
- Storage: Store according to the manufacturer's stated conditions on the product label and certificate of analysis
Handling and Storage
Store this material in its original tightly closed container, following the storage temperature stated on the manufacturer's label. Tetracycline compounds are sensitive to light and moisture, so containers should be kept closed, protected from light, and held in a dry location. Prepare aqueous stock solutions in clean, sterile containers and, where the workflow allows, filter-sterilize rather than heat-sterilize. Handle the powder in a fume hood or with suitable local exhaust to avoid inhaling dust, and wear gloves, safety glasses, and a laboratory coat. Avoid contact with skin and eyes. Dispose of residues and contaminated consumables as chemical or biological waste according to your institution's procedures, and consult the manufacturer's safety data sheet before use.
