2-Methyl-5-nitroimidazole-1-ethanol TCI M0924

TCI M0924 2-Methyl-5-nitroimidazole-1-ethanol, listed under CAS number 443-48-1, is a nitroimidazole compound widely known as metronidazole and recognized as one of the most important antimicrobial substances within the nitroimidazole group. Its core structure consists of an imidazole ring bearing a methyl group, a nitro group at the five position, and a hydroxyethyl side chain attached to the nitrogen atom. In the laboratory it serves as a reagent and test substance for antibiotic research, anaerobic microbiology and protozoal studies, and the development and validation of pharmaceutical analytical methods.

The property that makes this compound a preferred choice is its aromatic nitro group, which can undergo reduction under anaerobic conditions. This characteristic forms the basis of its selectivity toward anaerobic organisms and makes it a reference compound in studies of bioreductive prodrug mechanisms. The material is a crystalline solid that is easily weighed with accuracy, carries a strong chromophore so that it is readily detected by ultraviolet detectors, and shows adequate solubility in polar solvents such as methanol and dimethyl sulfoxide. The hydroxyl group on its side chain additionally provides a derivatization point for the synthesis of analogues or esters.

In Indonesian laboratories this material is used across university research groups, pharmaceutical quality control units, and microbiology facilities. It supports teaching and research work on antimicrobial agents, serves as a comparison substance in analytical method development, and is applied in studies involving anaerobic bacteria and protozoa. Its stable solid form and straightforward handling make it practical for routine use in laboratories working under tropical ambient conditions.

• Antibiotic research: serves as a well-characterized nitroimidazole test substance for investigating antimicrobial activity, allowing researchers to work with a compound whose behaviour in this class is already firmly established.
• Anaerobic microbiology studies: its nitro group is reduced under anaerobic conditions, making it directly suitable for experiments examining selectivity of antimicrobial action against oxygen-intolerant organisms.
• Protozoal research: the compound is applied as a reagent and test substance in protozoal investigations, where nitroimidazole chemistry is central to the biological questions being studied.
• Pharmaceutical analytical method development and validation: the strong chromophore gives reliable ultraviolet detection, and the crystalline solid form permits accurate weighing for preparing calibration and reference solutions.
• Bioreductive prodrug mechanism studies: as a reference compound for this mechanism, it provides a dependable model system for teaching and investigating reduction-dependent activation chemistry in the laboratory.

Store the container tightly closed in a cool, dry area protected from direct sunlight and away from sources of heat, following the storage directions supplied by the manufacturer. Amber glass bottles or the original supplier container are suitable, as the compound carries a strong chromophore and is better kept away from light. Handle in a well-ventilated area or a fume hood when weighing the solid, and wear a laboratory coat, gloves, and safety glasses. Avoid generating dust, close the container immediately after use to limit moisture uptake, and keep prepared solutions in methanol or dimethyl sulfoxide clearly labelled with the preparation date. Consult the manufacturer safety data sheet before first use and dispose of residues through the laboratory chemical waste route.

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