Bucladesine Sodium Salt TCI D4228

Name: TCI D4228 16980-89-5 Bucladesine Sodium Salt
Brand: TCI
SKU: TCI2510D422824764
Price: 2628150.0 IDR
Availability: InStock

Rp 2.628.150,00

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Berat 25mg

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Description

TCI D4228 Bucladesine Sodium Salt (CAS 16980-89-5) is the sodium salt of dibutyryl cyclic AMP, an analogue of cAMP whose two butyryl groups make the molecule far more readily able to cross the cell membrane than natural cAMP. In cell biology and biochemistry laboratories, this compound is used as a pharmacological tool to raise the activity of cAMP-dependent signalling pathways from outside the cell, without first having to stimulate adenylate cyclase. Researchers can therefore test directly the role of cAMP in differentiation, secretion, gene expression, and a wide range of other cellular responses.

The property that has made this reagent a standard in many protocols is the combination of membrane permeability and relative resistance to hydrolysis by phosphodiesterase. The butyryl groups mask the charge and add lipophilicity so that the molecule crosses the plasma membrane, after which intracellular esterases release it gradually, so that activation of protein kinase A is more sustained than with direct administration of cAMP. The sodium salt form dissolves well in aqueous media, making it straightforward to prepare sterile stock solutions that are practical to use on cell cultures.

In Indonesian laboratories, the reagent is typically found in university and institutional cell culture facilities, biochemistry and molecular biology teaching laboratories, and research groups working on cell signalling. It is generally handled at the bench as a stock solution prepared in aqueous medium and added to culture at the working concentration set by the protocol in use, alongside the other tissue culture reagents already established in the laboratory workflow.

Laboratory Applications

Cell signalling studies: raises cAMP-dependent pathway activity directly from outside the cell, so the role of cAMP can be tested without stimulating adenylate cyclase first.
Cell differentiation experiments: its sustained activation of protein kinase A makes it suitable for following differentiation responses that depend on prolonged cAMP signalling rather than a brief pulse.
Gene expression research: allows cAMP-dependent transcriptional responses to be induced in culture in a controlled way, since the compound enters cells readily and acts gradually.
Secretion and cellular response assays: the membrane-permeable analogue lets researchers examine secretory and other cellular responses that are governed by the cAMP second messenger system.
Enzyme and enzyme-inhibitor work: its relative resistance to phosphodiesterase hydrolysis makes it a dependable tool compound in biochemical studies of cAMP-related enzyme activity.

Specifications

Brand: TCI
CAS number: 16980-89-5
Category: Life Science > Biochemicals and Reagents > Enzymes, Enzyme Inhibitors, Enzyme Substrates
Chemical form: sodium salt of dibutyryl cyclic AMP (cAMP analogue), soluble in aqueous media
Pack sizes: available in the catalogue pack sizes offered for this TCI item; please refer to the current listing
Storage: follow the storage conditions stated on the manufacturer's label and certificate of analysis

Handling and Storage

Store the container tightly closed in the conditions indicated on the manufacturer's label, keeping the material dry and protected from moisture and light. Keep it in its original container or in a clean, well-sealed, chemically compatible vessel, and label any prepared stock solution clearly with its contents and preparation date. Prepare aqueous stock solutions under aseptic conditions when the material is intended for cell culture use, and aliquot them to avoid repeated handling of a single vessel. Handle the reagent in a well-ventilated area or a suitable cabinet, wearing gloves, safety glasses, and a laboratory coat, and avoid contact with skin, eyes, and clothing. Consult the manufacturer's safety data sheet before use, and dispose of residues and contaminated consumables through the laboratory's established chemical waste procedure.