TCI D5394 Dorsomorphin is a small-molecule organic compound widely used as a signalling pathway inhibitor in cell biology research. The compound is well known among researchers as an inhibitor of AMP-activated protein kinase and, at the same time, as an inhibitor of bone morphogenetic protein pathway signalling. In the laboratory, its role is that of a molecular tool: a substance deliberately added to cell cultures in order to shut down one specific biochemical pathway, so that researchers can observe the consequences for the growth, differentiation, or survival of the cells under study.
The characteristic that makes this compound a preferred choice is its nature as a small molecule capable of crossing the cell membrane. Researchers therefore need only add it to the culture medium, without resorting to complicated and expensive transfection techniques. Its effect is rapid and can be tuned through the concentration applied and the duration of exposure, in contrast to genetic approaches, which are generally permanent. The compound is customarily dissolved first in dimethyl sulfoxide to form a concentrated stock solution, which is then diluted into the medium before use.
Because this compound affects more than one pathway, researchers ordinarily interpret their results with that in mind and design their experiments accordingly. In Indonesian laboratories, the material is used in cell biology and cancer research settings where cultured cells are treated with a defined inhibitor and the resulting phenotype is compared against untreated controls. It is handled as a research chemical within standard cell culture practice, prepared as a stock solution and applied to the medium at the working concentration chosen by the investigator.
- Cancer research on cultured cell lines, where a membrane-permeable small molecule allows a specific signalling pathway to be shut down and the effect on cell growth or survival observed directly.
- Bone morphogenetic protein pathway studies, since this compound inhibits BMP pathway signalling and lets investigators examine how loss of that signal alters differentiation in the cells under study.
- AMP-activated protein kinase investigations, where the compound serves as a well-recognised AMPK inhibitor for probing the role of this kinase in cellular metabolic and survival responses.
- Cell differentiation experiments, because the depth and duration of inhibition can be tuned through concentration and exposure time rather than being fixed permanently as in genetic knockout approaches.
- Comparative pathway screening in cell culture, where the compound is added directly to the medium as a molecular tool, avoiding the cost and complexity of transfection-based methods.
| Brand | TCI |
|---|---|
| CAS number | 866405-64-3 |
| Molecular formula | — |
| Purity | — |
| Category | Life Science > Cell Biology > Cancer Research |
| Pack sizes | available in the pack sizes offered by TCI for this catalogue item; please confirm the required size when ordering |
| Physical form | — |
| Storage | — |
- Product code: D5394
- Form and handling: small-molecule research chemical, commonly dissolved in dimethyl sulfoxide to prepare a concentrated stock solution
Store this product according to the conditions stated on the manufacturer's label and safety data sheet, keeping the container tightly closed and protected from moisture and light. Stock solutions prepared in dimethyl sulfoxide should be kept in clean, well-sealed, chemically compatible vials, clearly labelled with the compound name, concentration, solvent, and preparation date, and aliquoted to limit repeated freeze–thaw cycles. Handle the material as a research chemical intended for laboratory use only, wearing a laboratory coat, gloves, and eye protection, and weigh or dispense it in a fume hood or suitable containment. Prepare dilutions into culture medium using aseptic technique, and dispose of residues and contaminated consumables in accordance with the laboratory's chemical waste procedures.
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