Erythromycin TCI E0751

Description

TCI E0751 Erythromycin is a macrolide-class antibiotic built around a fourteen-membered lactone ring bearing two attached sugars, one an amino sugar and one neutral. In the laboratory setting, this compound is not used as a medicine but rather as a standard biochemical and microbiological reagent. Researchers employ it to inhibit the growth of specific bacteria under controlled conditions, as a selection agent in expression systems, and as a reference compound in antimicrobial susceptibility testing and the development of instrumental analytical methods.

The principal property that makes erythromycin such a widely chosen reagent is its specific mode of action: it binds the 50S ribosomal subunit and disrupts the translocation step of bacterial protein synthesis. Its activity spectrum, which is particularly pronounced against Gram-positive bacteria, makes it a useful tool for establishing selective culture conditions. In addition, its complex macrolide framework has made erythromycin a classic reference compound in structure-activity relationship studies, investigations of antimicrobial resistance, and the development of semi-synthetic macrolide derivatives. Because it is supplied as a solid that can be weighed accurately, stock solutions are straightforward to prepare and dilute as required.

In Indonesian laboratories, this material is typically found in microbiology, biotechnology, and pharmaceutical analysis work. University research groups and molecular biology facilities use it to maintain selective pressure in culture work, while analytical and quality-control laboratories draw on it as a reference substance when establishing or verifying methods. Its solid form suits routine gravimetric handling on the bench, and it fits naturally into susceptibility testing workflows and media preparation performed as part of everyday laboratory practice.

Laboratory Applications

• Selective culture preparation — added to growth media to suppress susceptible bacterial populations, allowing researchers to establish controlled selective conditions where only the intended organisms proliferate.
• Selection agent in expression systems — used to maintain selective pressure on host cells carrying resistance markers, ensuring that only successfully transformed cultures continue to grow during propagation.
• Antimicrobial susceptibility testing — serves as a reference antibiotic against which the sensitivity of bacterial isolates is measured, giving laboratories a well-characterised macrolide benchmark for comparison.
• Analytical method development — employed as a reference compound when building and verifying instrumental analytical procedures, since its complex macrolide structure provides a demanding and informative test analyte.
• Structure-activity and resistance studies — its classic fourteen-membered macrolide framework makes it a standard reference point for investigating antimicrobial resistance mechanisms and designing semi-synthetic macrolide derivatives.

Specifications

• Brand: TCI (Tokyo Chemical Industry)
• CAS Number: 114-07-8
• Catalogue Number: E0751
• Category: Life Science > Biochemicals and Reagents > Antibiotics
• Physical Form: Solid, suitable for accurate weighing and stock solution preparation
• Pack Sizes: Available in the standard research pack sizes offered for this catalogue item; please confirm the size required when ordering
• Storage: Store according to the conditions stated on the manufacturer's label and product documentation

Handling and Storage

Store this reagent in its original tightly closed container, following the storage conditions specified on the manufacturer's label and in the accompanying product documentation. Keep the container in a dry, well-ventilated area away from moisture, direct sunlight, and sources of heat, and reseal it promptly after each use to preserve the integrity of the solid. Weigh the material in a clean, dedicated area and prepare stock solutions in appropriate chemically resistant labware, labelling all dilutions clearly with contents and date. Standard laboratory precautions apply: wear a laboratory coat, suitable gloves, and eye protection, avoid generating or inhaling dust, handle powders in a fume hood or ventilated enclosure where practicable, and wash hands thoroughly after handling. Dispose of surplus material and antibiotic-containing media in accordance with institutional and local waste regulations, and consult the Safety Data Sheet before first use.