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Description
TCI C2227 Ciprofloxacin Hydrochloride Monohydrate is the hydrochloride monohydrate salt of ciprofloxacin, a fluoroquinolone-class antibiotic that has become one of the most widely used microbiological reagents in research worldwide. The compound acts by inhibiting bacterial DNA gyrase and topoisomerase IV, halting replication of genetic material so that bacterial cells fail to multiply. Because this mechanism differs fundamentally from that of beta-lactam antibiotics, ciprofloxacin serves as an important comparator in susceptibility testing panels, cross-resistance research, and molecular-level studies of antibacterial drug action in microbiology and pharmaceutical laboratories.
The hydrochloride monohydrate salt form provides considerably better aqueous solubility than the free base, making the preparation of stock solutions for culture media and assay dilution series both practical and rapid. The fixed water of crystallisation content supports accurate molar concentration calculations, which matters whenever a dilution series must be reproducible across runs. Its broad activity spectrum, particularly against Gram-negative bacteria, makes it an effective choice for selective media and for clearing cell cultures of mycoplasma and bacterial contamination. The fluoroquinolone character of the molecule also underpins its consistent behaviour as a reference antibacterial agent.
In Indonesian laboratories, this reagent is routinely used across university microbiology departments, pharmaceutical quality control units, and research institutes engaged in antimicrobial resistance work. It supports teaching laboratories running susceptibility demonstrations, cell culture facilities maintaining contamination-free lines, and formulation groups requiring a dependable fluoroquinolone standard. Supplied under the TCI brand through AMI Scientific, it fits established laboratory routines where a well-characterised, readily soluble antibiotic is needed for daily microbiological and pharmaceutical work.
Laboratory Applications
Antimicrobial susceptibility testing: serves as a standard fluoroquinolone comparator in disc diffusion and broth dilution panels, giving laboratories a consistent reference point for interpreting isolate response.
Cross-resistance and resistance mechanism research: its distinct gyrase and topoisomerase IV target set allows researchers to distinguish fluoroquinolone resistance patterns from beta-lactam resistance in the same bacterial collection.
Selective culture media preparation: the broad Gram-negative spectrum lets media developers suppress unwanted background flora while permitting growth of the target organisms under investigation.
Cell culture decontamination: effective against mycoplasma and bacterial contaminants, it helps rescue valuable cell lines without requiring the culture to be discarded and re-established from frozen stock.
Molecular mechanism of action studies: the well-defined fluoroquinolone target interaction makes it a suitable probe compound for investigating how antibacterial agents interfere with DNA replication machinery.
Specifications
Brand: TCI
CAS number: 86393-32-0
Category: Life Science > Biochemicals and Reagents > Antibiotics
Chemical form: hydrochloride monohydrate salt of ciprofloxacin, with fixed water of crystallisation
Pack sizes: available in the standard catalogue pack sizes offered by TCI for this catalogue number
Storage note: store according to the manufacturer's stated conditions on the product label and safety data sheet
Handling and Storage
Store the container tightly closed in a cool, dry place away from direct light, following the storage conditions stated on the manufacturer's label and safety data sheet. Keep the material in its original container or in a clean, well-sealed amber glass or chemically compatible vessel, clearly labelled with the compound name and CAS number. Handle in a well-ventilated area or fume hood, wearing gloves, a laboratory coat, and eye protection, and avoid generating dust during weighing. Prepared aqueous stock solutions should be labelled with the preparation date and handled aseptically when intended for culture media. Allow sealed containers to reach room temperature before opening to prevent moisture condensation, and dispose of residues in accordance with institutional chemical waste procedures.
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