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Description
TCI G0226 L-(-)-Glucose is the left-handed enantiomeric form of glucose, the mirror image of the D-glucose that occurs commonly in nature and serves as the primary energy source for living organisms. Because its spatial configuration is reversed, this compound is not recognized by most enzymes and cellular transport systems, which act stereospecifically. In the laboratory, this very property is what makes L-glucose so valuable: it serves as an "inert" comparator that is structurally almost identical to D-glucose, allowing researchers to separate genuinely metabolic effects from physical effects such as the osmolarity or viscosity of a solution.
The principal characteristic that leads researchers to select L-(-)-glucose is its stereochemical purity. As a rare sugar that is not abundantly available in nature, this material is generally produced through directed synthesis and tightly controlled so that it is not contaminated by the D enantiomer, which would undermine the validity of an experiment. Physically the compound is a white crystalline solid that dissolves well in water, so it is easily prepared as a stock solution or added directly to culture medium. Its negative optical rotation provides a practical means of verifying identity.
In Indonesian laboratories, this material is used in academic and institutional research settings where stereospecific control experiments are required — university biochemistry and cell biology groups, molecular biology laboratories, and research units working on glucose transport and metabolism. It is typically handled as a small-quantity reference reagent rather than a bulk chemical, weighed out for preparation of defined stock solutions and stored alongside other sensitive research-grade materials in a controlled reagent store.
Laboratory Applications
Osmotic control in cell culture experiments, where L-glucose matches the osmolarity contributed by D-glucose without being metabolised, isolating true metabolic effects.
Glucose transport and uptake studies, in which the stereospecificity of transporters means L-glucose is excluded, providing a baseline for non-specific or passive movement.
Enzyme specificity investigations, where L-glucose is offered as a substrate analogue to confirm that a carbohydrate-active enzyme discriminates strictly between the two enantiomers.
Stereochemistry teaching and method development, since the negative optical rotation of L-glucose gives a clear, practical demonstration and identity check by polarimetry.
Formulation of control media and buffers, where a structurally near-identical but biologically inert sugar is needed to maintain viscosity and solution properties without supplying energy.
Specifications
Brand: TCI (Tokyo Chemical Industry)
CAS number: 921-60-8
Category: Chemistry > Chemical Biology > Glycoscience
Catalogue number: G0226
Physical form: white crystalline solid, readily soluble in water
Pack sizes: available in the research-scale pack sizes listed for this catalogue item
Storage: keep in a tightly closed container in a cool, dry place, protected from moisture
Handling and Storage
Store L-(-)-glucose in its original tightly closed container in a cool, dry, well-ventilated place away from moisture, since crystalline sugars readily absorb atmospheric water and may cake or lose free-flowing character. Use clean, dry glass or chemically compatible plastic containers for weighing and for prepared stock solutions, and label them clearly to avoid confusion with D-glucose, as the two are visually indistinguishable. Handle with standard laboratory precautions: wear a laboratory coat, gloves and eye protection, weigh in a clean area to avoid cross-contamination that would compromise stereochemical purity, and close the container promptly after each use. Prepared aqueous solutions should be stored under conditions appropriate to the experiment and protected from microbial contamination.
