D-(+)-Lactose Monohydrate is a milk disaccharide composed of galactose and glucose, crystallised in a hydrated form that carries one molecule of water. The product is supplied as a solid and ranks among the most classical and most frequently used materials found in biology, microbiology, and pharmaceutical laboratories. Its role is unusually broad: it serves as a carbon source in microbial growth media, as a substrate for fermentation tests and lactase enzyme assays, and as a long-established filler in the development of solid dosage preparations. Because it is such a fundamental material, it is normally kept permanently on the routine reagent shelf.
The properties that make lactose monohydrate a preferred choice are its crystal stability, its good flow behaviour in powder form, its solubility in water, and the reducing capacity it possesses as a reducing sugar. The stability of the monohydrate form makes consistent weighing straightforward from one day to the next, without meaningful changes in mass over time. In microbiological media, the fact that some microorganisms are able to ferment lactose while others are not turns the material into a highly useful differential marker. Its chemical character is also relatively inert towards many active substances, which broadens the range of formulations in which it can be placed.
In Indonesian laboratories this material sits in the everyday working stock rather than in the specialist cabinet. It is drawn on whenever growth media are prepared, whenever fermentation behaviour needs to be distinguished between bacterial isolates, and whenever a dependable solid diluent or filler is required during preparation development. Teaching laboratories, microbiology service laboratories, and pharmaceutical development units all tend to keep it available, precisely because so many routine procedures assume it is already on hand.
- Microbial growth media preparation — lactose monohydrate supplies the carbon source that supports microbial growth, and its water solubility allows it to be dissolved cleanly into media before use.
- Fermentation testing and differential identification — because certain microorganisms ferment lactose while others cannot, the sugar acts as a reliable differential marker when isolates are being distinguished from one another.
- Lactase enzyme assays — the material serves directly as the substrate for lactase activity work, giving a defined disaccharide on which the enzyme's hydrolytic behaviour can be observed.
- Solid dosage formulation development — its long-established use as a filler, combined with good powder flow, makes it a practical bulking material during the development of solid preparations.
- Reducing sugar work and routine reagent use — its reducing capacity as a reducing sugar supports chemistry involving reducing sugars, and its constant shelf presence makes it convenient for everyday procedures.
| Brand | TCI |
|---|---|
| CAS number | 64044-51-5 |
| Molecular formula | — |
| Purity | — |
| Category | Chemistry > Chemical Biology > Glycoscience |
| Pack sizes | available in the pack sizes listed on this product page |
| Physical form | solid, crystalline monohydrate (one molecule of water of crystallisation) |
| Storage | store in a closed container under normal laboratory reagent storage conditions |
Store D-(+)-Lactose Monohydrate in a tightly closed container, kept dry and away from moisture so that the crystalline monohydrate form and its associated weighing consistency are preserved. Original supplier packaging or a clean, well-sealed glass or plastic reagent container is suitable, clearly labelled with the material name and CAS number. Handle the powder in a well-ventilated area and avoid generating unnecessary dust during weighing and transfer. Use clean, dry spatulas to prevent contamination of the bulk stock, and close the container promptly after each use. Standard laboratory personal protective equipment — a laboratory coat, gloves, and eye protection — should be worn, and general good laboratory practice for handling solid chemical reagents should be followed. Consult the supplier safety data sheet before first use.
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