Europium Chelate Reagent to Form Fluorescent Labels for Time-Resolved Fluorometry
ATBTA-Eu3+ (A2083) is a europium chelate complex used as a fluorescent labeling reagent. After conversion to DTBTA-Eu3+ with Cyanuric Chloride (C0460), the reagent is easily labeled to protein. Its fluorescence is stable in various aqueous buffers and it is available in Tris, TE, and PBS. Its advantages include no cross talk of excitation light, a sharpened emission spectrum, a large Stokes shift, and a long fluorescence lifetime (1.02 ms) for time-resolved fluorometry. DTBTA-Eu3+ conjugates also support sensitive detection of biotinylated antibodies and various probes, and tag-based immunological detection by ELISA and Western blotting. In a typical procedure, 2 mg ATBTA-Eu3+ is converted using 0.43 mg Cyanuric Chloride in 25 µL acetone. In the brochure comparison chart, the non-antigen background of the DTBTA-Eu3+ conjugate is much lower than that of FITC. On a TCI practical example page, Cyanuric Chloride (C0460) is also used to dehydrate 4-bromobenzamide to 4-bromobenzonitrile in 95% yield.
Related products
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Download TCI brochure Europium Fluorescent Labeling Reagent and Labeled Probes (PDF)
Source
- TCI Practical Example: www.tcichemicals.com/ID/en/product/tci-topics/TCIPracticalExample_20241021
- TCI product page A2083: tcichemicals.com/ID/en/p/A2083
- TCI product page C0460: tcichemicals.com/ID/en/p/C0460
- TCI brochure LL001: Europium Fluorescent Labeling Reagent and Labeled Probes
For research and laboratory use only, not for human consumption.
