N-Succinimidyl 6-Biotinamidohexanoate TCI S0490

Chemical Identity

CAS No.: 72040-63-2
PubChem: CID 83874·SID 253659648
Formula: C20H30N4O6S
Molecular weight: 454.5 g/mol
Purity: >97.0%(HPLC)
Reference databases: ECHA

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate
SMILES: C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCC[C@H]2[C@@H]3[C@H](CS2)NC(=O)N3
InChIKey: UVGHPGOONBRLCX-NJSLBKSFSA-N

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N-Succinimidyl 6-Biotinamidohexanoate (SBH), also known as TCI S0490 72040-63-2, is a chemical compound widely used in bioconjugation applications within laboratory settings. It serves as a key reagent for attaching biotin to various biomolecules such as proteins, nucleic acids, and other functional groups. This compound plays a critical role in molecular biology and biochemical research by enabling the creation of biotinylated molecules with high specificity and efficiency. Its utility spans multiple experimental techniques, making it an essential tool for scientists working in biotechnology and life sciences.

SBH is preferred due to its high reactivity towards amino groups and its chemical stability under controlled conditions. The compound’s ability to form strong, covalent bonds with target molecules ensures reliable and reproducible results in experimental settings. Its high purity further enhances the accuracy of research outcomes, making it a trusted reagent in both academic and industrial laboratories. Additionally, SBH exhibits resistance to extreme environmental conditions, allowing for greater flexibility in experimental design and application.

In Indonesian laboratories, SBH is commonly used in biochemical and molecular biology research. It is a staple in institutions conducting studies related to biotinylation, immunodetection, and protein labeling. Its versatility and effectiveness make it a valuable resource for researchers aiming to develop and refine techniques such as ELISA, Western blot, and other biotin-based assays. Its consistent performance and reliability contribute to the advancement of scientific research in the region.

TCI brochures (PDF)

Europium Fluorescent Labeling Reagent and Labeled Probes 2025-01-30 · p. 4
Streptavidins and Related Reagents 2025-02-28 · p. 3
Biotinylation Reagents and Avidins 2025-06-26 · p. 1
Bioconjugation Reagents 2026-07-07 · p. 2
Reagents for Biological Research 2025-03-11 · p. 15

Related TCI products

ELISA (Enzyme-Linked Immunosorbent Assay) utilizes SBH to biotinylate antibodies, enhancing signal detection and specificity in immunoassays.
Western blot analysis benefits from SBH’s ability to conjugate biotin to proteins, enabling efficient detection through streptavidin-based systems.
Biotinylation of proteins is a common application where SBH allows for the covalent attachment of biotin to amino groups, facilitating downstream purification and analysis.
Molecular biology research often employs SBH to label nucleic acids, aiding in hybridization studies and gene expression analysis.
Immunohistochemistry applications use SBH to biotinylate antibodies, improving the sensitivity and accuracy of tissue staining techniques.

Brand	TCI
CAS number	72040-63-2
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Bioconjugation
Pack sizes	Available in various quantities as per standard laboratory reagent packaging
Physical form	Solid powder
Storage	Store at room temperature, away from moisture and direct sunlight

SBH should be stored in a cool, dry place, away from direct sunlight and sources of heat. It is recommended to use airtight containers to prevent moisture absorption, which could affect its chemical stability. In laboratory settings, it is important to handle SBH with appropriate personal protective equipment, such as gloves and safety goggles, to ensure safe handling. The compound should be kept in a well-ventilated area to minimize exposure to vapors. Due to its reactivity, it is advisable to avoid prolonged contact with skin or eyes. Proper storage and handling protocols help maintain the integrity and effectiveness of SBH in research applications.

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