Di(N-succinimidyl) Sebacate TCI D4019

Name: TCI D4019 23024-29-5 Di(N-succinimidyl) Sebacate
Brand: TCI
SKU: TCI2510D401924483
Price: 1800750.0 IDR
Availability: InStock

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Berat 1g 5g

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Description

Di(N-succinimidyl) Sebacate is a homobifunctional crosslinking reagent carrying two N-hydroxysuccinimide (NHS) ester groups at either end of a ten-carbon sebacate chain. The NHS ester groups react with primary amines — principally the amino groups of lysine residues and the N-terminus of a protein chain — to form stable amide bonds. Because both ends of the molecule are reactive toward the same functional group, the reagent is used to join two protein-bearing molecules together, to immobilise biomolecules onto solid surfaces, or to lock protein–protein interactions in place so that they can be analysed further in the chemical biology laboratory.

The advantage that makes this reagent a preferred choice lies in the length of its spacer arm. The sebacate chain provides a more generous distance between the two points of attachment than short-arm crosslinkers do, which makes it well suited to bridging amine groups that are located far apart from one another, or to keeping an immobilised biomolecule free enough to move so that it does not lose its function. That long aliphatic chain is hydrophobic in character and dissolves well in organic solvents such as dimethyl sulfoxide or dimethylformamide, so stock solutions are normally prepared in one of those solvents before being added to the reaction.

In Indonesian laboratories, this type of reagent is typically handled in university research groups, biotechnology institutes, and protein chemistry facilities working on conjugation and bioimmobilisation. The usual working pattern is to prepare a concentrated stock in dimethyl sulfoxide or dimethylformamide first, then introduce a small volume of that stock into the aqueous protein solution being crosslinked. Because the reagent is supplied by TCI and is used in small quantities per experiment, it is normally kept as a shared stock item within the laboratory.

Laboratory Applications

Protein–protein conjugation: the two NHS ester ends react with lysine and N-terminal amines on separate protein molecules, joining them through stable amide bonds.
Immobilisation of biomolecules on solid supports: one NHS ester anchors to an amine-functionalised surface while the other captures the biomolecule to be tethered.
Capture of protein–protein interaction partners: interacting proteins are locked together covalently so that the complex survives subsequent analytical steps in the laboratory.
Long-spacer crosslinking studies: the ten-carbon sebacate chain bridges amine groups that lie too far apart for short-arm crosslinkers to reach effectively.
Preparation of functionally active immobilised proteins: the generous arm length preserves conformational freedom, helping tethered biomolecules retain their biological function after attachment.

Specifications

Brand: TCI
CAS number: 23024-29-5
Catalogue reference: D4019
Category: Chemistry > Chemical Biology > Linkers and Crosslinkers
Pack sizes: available in standard TCI research-scale packaging; please confirm the size required when ordering
Storage note: keep tightly closed and protected from moisture, since NHS esters are susceptible to hydrolysis

Handling and Storage

Store the container tightly sealed in a cool, dry place and protect it from moisture, because the NHS ester groups are hydrolysed by water and lose reactivity once that happens. Keep the reagent in its original tightly closed container, and allow it to reach ambient temperature before opening so that condensation does not form inside. Prepare stock solutions in dry dimethyl sulfoxide or dimethylformamide immediately before use rather than storing dilute aqueous solutions. Handle the material in a fume hood while wearing gloves, safety glasses, and a laboratory coat, avoid contact with skin and eyes, and consult the manufacturer's safety data sheet before beginning work.