Donors and Acceptors for FRET

Donors and Acceptors for FRET — contains fluorophore donor-acceptor pairs, including coumarin, dansyl, and azo dye derivatives with succinimidyl ester substitution, selected based on emission-excitation spectral overlap for fluorescence resonance energy transfer. The NHS active ester form in many members facilitates covalent conjugation to target biomolecules.

These FRET donor-acceptor reagents are used by molecular biology researchers to label proteins, peptides, or oligonucleotides to monitor close-range interactions, conformational changes, or enzyme activity in real time based on changes in fluorescence signal. Pairs such as coumarin and azo dye derivatives are selected based on appropriate spectral overlap, while probes such as 6-HEX are commonly used in primer or probe design for fluorescence-based PCR techniques.

Examples of products in this collection: TCI S0891 100900-07-0 Sodium 5-(2-Aminoethylamino)-1-naphthalenesulfonate, TCI B6539 916753-62-3 2,5-Dioxopyrrolidin-1-yl 4-[[4-[[2,5-Dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]phenyl](methyl)amino]butanoate, TCI S0867 150321-92-9 N-Succinimidyl 7-Methoxycoumarin-3-carboxylate, TCI B6557 374591-94-3 2-[[2-[Bis(4-methoxyphenyl)(phenyl)methoxy]ethyl][4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)diazenyl]phenyl]diazenyl]phenyl]amino]ethyl (2-Cyanoethyl) Diisopropylphosphoramidite, TCI S0866 134471-24-2 N-Succinimidyl 7-Hydroxycoumarin-3-carboxylate, TCI B6558 374591-98-7 2-[[2-[Bis(4-methoxyphenyl)(phenyl)methoxy]ethyl][4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]phenyl]amino]ethyl (2-Cyanoethyl) Diisopropylphosphoramidite, TCI S0857 146998-31-4 N-Succinimidyl 4-[4-(Dimethylamino)phenylazo]benzoate, TCI B6559 3-[[4-[[2-[Bis(4-methoxyphenyl)(phenyl)methoxy]ethyl][2-[[(2-cyanoethoxy)(diisopropylamino)phosphaneyl]oxy]ethyl]amino]phenyl]diazenyl]-7-(diethylamino)-5-phenylphenazin-5-ium Hexafluorophosphate(V).

Select donor-acceptor pairs based on adequate emission-excitation spectral overlap and the type of reactive groups (NHS ester, amine) that match the conjugation site on the target biomolecule. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories aligned under Fluorescent Probes, Fluorescent Labels, Fluorescent Stains, are often used together in one laboratory workflow:

Back to Fluorescent Probes, Fluorescent Labels, Fluorescent Stains. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official TCI Japan distributor in Indonesia — for availability checks and lead time directly from Japan.

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