Collection: Media Components [Molecular Biology and Protein Expression]

Media Components [Molecular Biology and Protein Expression] — contains chromogenic and luminogenic substrates such as X-gal, ONPG, and luciferin derivatives that react with specific reporter enzymes to produce measurable color or light. These materials serve as supporting components in culture media and enzyme-reporter-based gene expression assays.

Media Components are widely used in molecular biology for screening bacterial colonies through blue-white screening assays using X-gal, as well as in beta-galactosidase activity assays using ONPG and nitrophenyl derivative substrates. D-Luciferin is used by researchers in bioluminescence to measure luciferase reporter gene expression in living cell systems and model animals.

Example products in this collection: TCI B3470 97753-82-7 5-Bromo-3-indolyl beta-D-Galactopyranoside [for Biochemical Research], TCI C2371 138182-21-5 6-Chloro-3-indolyl beta-D-Galactopyranoside [for Biochemical Research], TCI B3201 7240-90-6 5-Bromo-4-chloro-3-indolyl beta-D-Galactopyranoside [for Biochemical Research], TCI N0418 369-07-3 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase], TCI A5030 2591-17-5 D-(-)-Luciferin [Chemiluminescence Reagent], TCI N0616 3150-24-1 4-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-Galactosidase].

Select substrates according to the reporter enzyme used in the expression system, and pay attention to the purity grade for biochemistry so that background signal in colorimetric or luminometric assays remains low. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under Enzyme Substrates [Biochemicals and Reagents], often used together in one laboratory workflow:

• Enzyme Inhibitors [Biochemicals and Reagents]
• Enzymes [by Application]
• Enzyme Class Index

Back to Enzyme Substrates [Biochemicals and Reagents]. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official TCI Japan distributor in Indonesia — to check availability and lead time directly from Japan.