N-Hydroxysuccinimide [Coupling Reagent for Peptide] TCI B0249

Chemical Identity

CAS No.: 6066-82-6
PubChem: CID 80170·SID 87563364
Formula: C4H5NO3
Molecular weight: 115.09 g/mol
Purity: >98.0%(GC)(T)
Reference databases: ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 1-hydroxypyrrolidine-2,5-dione
SMILES: C1CC(=O)N(C1=O)O
InChIKey: NQTADLQHYWFPDB-UHFFFAOYSA-N

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N-Hydroxysuccinimide (NHS) is a widely used coupling reagent in peptide synthesis and protein modification. As a covalent reagent, it plays a crucial role in forming amide bonds between amino groups of proteins or peptides and other molecules. This compound is essential in chemical and biological laboratories due to its ability to enable specific and efficient bonding between various organic molecules. In peptide chemistry, NHS is employed to activate carboxyl groups, facilitating bonding reactions with amino groups. Its chemical properties allow it to remain stable under certain conditions while being highly reactive toward amino groups. This reactivity makes NHS ideal for covalent reactions requiring high specificity. Additionally, NHS exhibits good reactivity without producing toxic byproducts, making it safe for use in laboratory settings. Due to these characteristics, NHS is a preferred choice in a variety of chemical and biochemical applications. In Indonesia, it is extensively used in molecular biology, pharmacology, and organic chemistry research. Researchers and local laboratories often rely on NHS for peptide synthesis and modification processes.

TCI brochures (PDF)

Condensing Agents 2025-11-18 · p. 4

Related TCI products

Peptide Synthesis: NHS is ideal for activating carboxyl groups, enabling efficient coupling with amino groups in peptide chains.
Protein Modification: It facilitates the covalent attachment of functional groups to proteins, enhancing their biological activity.
Bioconjugation Studies: NHS allows the specific and controlled linking of biomolecules, supporting research in molecular biology.
Drug Development: It is used in the synthesis of bioactive compounds, aiding in the creation of therapeutic agents.
Organic Chemistry Research: NHS supports the formation of amide bonds in synthetic organic reactions, expanding the range of chemical modifications.

Brand	TCI
CAS number	6066-82-6
Molecular formula	—
Purity	—
Category	Chemistry > Chemical Biology > Peptide Chemistry
Pack sizes	Available in various quantities as per standard laboratory requirements
Physical form	White crystalline solid
Storage	Store in a cool, dry place away from moisture and light

N-Hydroxysuccinimide should be stored in a cool, dry place, away from moisture and direct light to maintain its stability and reactivity. It is recommended to use airtight containers to prevent exposure to air and humidity, which may affect its performance. Since NHS is highly reactive toward amino groups, it should be handled with care in laboratory settings to avoid unintended chemical reactions. Proper personal protective equipment, such as gloves and safety goggles, should be worn when handling the compound. Storage conditions should be controlled to ensure the material remains effective for its intended applications. Regular monitoring of storage conditions is advised to maintain the integrity of the reagent.

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