Bis(1,3-dibutylbarbituric Acid) Trimethine Oxonol (DiBAC4(3)) is an anionic voltage-sensitive fluorescent dye widely employed in cell biology research for monitoring membrane potential changes. It enters depolarized cells and binds to intracellular components, producing a measurable fluorescence signal ideal for flow cytometry and fluorescence microscopy applications. This compound is commonly applied in hematology and histology studies involving cell viability assessment, platelet activation, and cellular response analysis.
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