TCI
Carbenicillin Disodium (mixture of isomers) TCI C2241
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Description
TCI C2241 Carbenicillin Disodium (mixture of isomers) is the disodium salt of carbenicillin, an extended-spectrum penicillin-class antibiotic supplied as a biochemical reagent for research purposes. The product is a mixture of isomers, as stated in its name. In molecular biology and microbiology laboratories, carbenicillin plays a very distinctive role: it is widely used as a selection agent in bacterial cultures carrying plasmids with beta-lactam resistance genes, and as a test substance in studies of antibiotic mechanisms of action against the bacterial cell wall.
The property that makes carbenicillin the preferred choice is its better stability compared with ampicillin in culture media, so that selective pressure is maintained for a longer period during incubation. This matters because declining selective pressure frequently gives rise to satellite colonies that interfere with cloning results. The disodium salt form provides excellent solubility in water, so stock solutions are easy to prepare and sterile filter. The powder is hygroscopic and heat sensitive, so proper handling has a direct bearing on its performance.
In Indonesian laboratories, this reagent belongs to the Life Science category under Biochemicals and Reagents > Antibiotics, and is used in the routine work of molecular biology and microbiology units. The 10 g pack size suits laboratories that prepare selection media regularly, including university research groups, biotechnology laboratories, and testing facilities that maintain plasmid-bearing bacterial cultures and carry out antibiotic mechanism studies as part of their ongoing programmes.
Laboratory Applications
- Plasmid selection in bacterial culture — carbenicillin selects for cells carrying beta-lactam resistance genes and holds selective pressure longer than ampicillin during incubation.
- Molecular cloning workflows — the sustained selective pressure suppresses satellite colonies that would otherwise interfere with the interpretation of cloning results on plates.
- Antibiotic mechanism of action studies — as a penicillin-class, extended-spectrum agent it serves as a test substance in research on the bacterial cell wall.
- Preparation of selective culture media — the disodium salt dissolves readily in water, so stock solutions are simple to prepare and sterile filter before addition.
- General microbiology research — the extended-spectrum profile of this penicillin-class antibiotic supports investigative work on bacterial cultures in research settings.
Specifications
- Brand: TCI
- CAS number: 4800-94-6
- Category: Life Science > Biochemicals and Reagents > Antibiotics
- Pack size: 10 g
- Physical form: powder (disodium salt, mixture of isomers)
- Storage note: hygroscopic and heat sensitive; store accordingly
Handling and Storage
Because the powder is hygroscopic and heat sensitive, store the container in a cool place away from heat sources and keep it tightly closed so that atmospheric moisture is not absorbed during storage. Keep the material in its original tightly sealed container, and allow a chilled container to reach room temperature before opening to reduce condensation on the powder. Weigh out quantities promptly and reseal without delay. Prepare aqueous stock solutions and sterile filter them for use in culture media. Handle in accordance with standard laboratory practice for research reagents, wearing appropriate personal protective equipment, and note that this product is intended for research use.
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