Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis] TCI E1363

Chemical Identity

Other names: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide (0.5mg/mL in Water) · Homidium Bromide (0.5mg/mL in Water)
CAS No.: 1239-45-8
PubChem: CID 14710·SID 468591733
Formula: C21H20BrN3
Molecular weight: 394.32 g/mol
Storage: 0-10°C
Reference databases: ChEBI·ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine bromide
SMILES: CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
InChIKey: ZMMJGEGLRURXTF-UHFFFAOYSA-N
MDL: MDL00011724

Rp 1.298.850,00

~~Rp 924.000,00~~ Rp 1.298.850,00

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Volume 10mL

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Ethidium Bromide (EB) is a widely used chemical in molecular biology laboratories for visualizing DNA during electrophoresis. It functions as a fluorescent stain that intercalates with DNA strands, enabling detection under UV light. This property makes it an essential tool for researchers analyzing DNA fragments separated by an electric field. Its strong fluorescent characteristics allow for clear observation of DNA bands on gels using gel documentation systems or UV detection equipment.

Ethidium Bromide is preferred for its high stability under laboratory conditions and its ability to provide clear, consistent results. Its fluorescent properties are highly sensitive, making it ideal for detecting even small amounts of DNA. The compound is commonly used in routine electrophoresis procedures due to its reliability and ease of use. Its solubility in water and compatibility with standard laboratory protocols further enhance its utility in various molecular biology applications.

In Indonesian laboratories, Ethidium Bromide is frequently used in genetic research, diagnostics, and biological analysis. It is particularly relevant for educational institutions, research centers, and clinical laboratories that rely on electrophoresis for DNA analysis. Its application accelerates the identification and analysis of DNA fragments, supporting a wide range of scientific investigations. Its presence in Indonesian labs underscores its importance in molecular biology and related fields.

Advantages (TCI brochure)

High sensitivity detection
User-friendly dropper bottle format
Requires a UV transilluminator or equivalent detection system

TCI brochures (PDF)

Ultra-High-Sensitivity Fluorescent Gel Staining Reagent Nucleic Acid Stain Green I 2026-06-25 · p. 1
Electrophoresis Reagents 2026-09-15 · p. 10
Protein and Biological Sample Preparation Reagents 2026-09-10 · p. 12
Reagents for Biological Research 2025-03-11 · p. 7

Related TCI products

Genetic Research: Ethidium Bromide is used to visualize DNA fragments during electrophoresis, aiding in the analysis of genetic material for research purposes.
Diagnostic Testing: It enables the detection of DNA in samples, supporting diagnostic applications in clinical and research settings.
Biological Analysis: The fluorescent properties of Ethidium Bromide allow for the clear observation of DNA structures in gel electrophoresis experiments.
Educational Institutions: It is a standard reagent used in teaching laboratories to demonstrate DNA visualization techniques to students.
Clinical Laboratories: Ethidium Bromide supports DNA analysis in diagnostic workflows, ensuring accurate and reliable results in clinical settings.

Brand	TCI
CAS number	1239-45-8
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	1239-45-8 Ethidium Bromide (0.5mg/mL in Water) in Dropper Bottle
Physical form	Liquid solution
Storage	Store at room temperature, away from light and heat

Data from the TCI brochure

Sensitivitas	a few ng
Waktu pewarnaan	15 - 30 minutes

Source: TCI brochure LL141 (2026-06-25)

Ethidium Bromide should be stored at room temperature in a cool, dark place to maintain its stability and effectiveness. It is recommended to use amber-colored containers to protect it from light exposure, which can degrade the compound over time. Due to its potential toxicity, it should be handled with care, using appropriate personal protective equipment such as gloves and safety goggles. Avoid direct contact with skin or inhalation of vapors. In laboratory settings, proper disposal procedures should be followed to prevent environmental contamination. Ethidium Bromide is a hazardous substance and must be used in well-ventilated areas to minimize exposure risks.

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