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Description
Ethyl beta-D-Glucopyranoside is a simple glycoside formed from D-glucose in its pyranose ring form, with an ethyl group attached to the anomeric carbon through a beta configuration. The compound belongs to the glycoscience category and serves as an important material in carbohydrate research. In the laboratory it is used primarily as a model substrate for studying enzymes that cleave glycosidic bonds, as a reference compound in glycoside analysis, and as a comparison standard in the development of glycoside synthesis methods that require stereochemical control at the anomeric center.
The properties that make this compound a preferred choice are its well-defined beta configuration at the anomeric carbon and its simple ethyl aglycone. Because the aglycone is small and carries no interfering functional groups, changes observed during an experiment can be attributed directly to cleavage of the glycosidic bond rather than to other side reactions. The four free hydroxyl groups on the pyranose ring make the material highly soluble in water and polar solvents, while at the same time providing reaction points for selective protection, acylation, or further modification in carbohydrate synthesis research.
In Indonesian laboratories this material is typically handled in university chemistry and biochemistry departments, government research institutes, and analytical service laboratories working on carbohydrate and enzyme topics. It is generally used at small scale in benchtop enzyme assays, method development work, and student research projects, where a defined and well-characterised glycoside is needed as a starting point before moving on to more complex or less readily available carbohydrate substrates.
Laboratory Applications
Glycosidase enzyme assays: the defined beta linkage gives a clean, predictable substrate so that measured activity reflects genuine glycosidic bond cleavage rather than background reactions.
Reference compound in glycoside analysis: its known structure and simple aglycone make it a dependable comparison point when identifying or confirming glycoside peaks in analytical work.
Glycoside synthesis method development: researchers use it as a benchmark product when evaluating coupling methods that must deliver stereochemical control at the anomeric centre.
Selective protection and derivatisation studies: the four free hydroxyl groups on the pyranose ring provide practical reaction sites for testing protecting group strategies and acylation conditions.
Teaching and training in carbohydrate chemistry: the compound illustrates anomeric configuration and glycosidic bonding clearly, making it well suited to instructional laboratory exercises and student research.
Specifications
Brand: TCI
Catalogue number: E1643
CAS number: 3198-49-0
Category: Chemistry > Chemical Biology > Glycoscience
Pack sizes: available in standard TCI research pack sizes; please confirm the size required when ordering
Storage: keep in a cool, dry place in the original tightly closed container
Handling and Storage
Store the container in a cool, dry, well-ventilated area away from direct sunlight, moisture, and sources of heat. Keep the material in its original tightly closed container, or in a clean glass bottle with a secure cap, to prevent moisture uptake, since the free hydroxyl groups make the compound readily soluble in water and sensitive to humid storage conditions. Handle the material in a well-ventilated laboratory area or fume hood while wearing a laboratory coat, safety glasses, and suitable gloves. Use clean, dry spatulas when weighing to avoid contaminating the stock, and close the container immediately after use. Label any prepared solutions clearly with the compound name and preparation date, and follow the institutional chemical waste procedures for disposal of residues.
