• 2508-19-2 Picrylsulfonic Acid (ca. 1% in N,N-Dimethylformamide) [for Detection of Primary Amines] - AMI Scientific
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TCI

CAS 2508-19-2

Picrylsulfonic Acid (ca. 1% in N,N-Dimethylformamide) [for Detection of Primary Amines] TCI P1447

Picrylsulfonic Acid (ca. 1% in N,N-Dimethylformamide) [for Detection of Primary Amines] TCI P1447

Chemical Identity

CAS No.
2508-19-2
PubChem
CID 11045·SID 87575516
Formula
C6H3N3O9S
Molecular weight
293.17 g/mol
Reference databases
ChEBI·ChemSpider·ECHA·Wikipedia
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
2,4,6-trinitrobenzenesulfonic acid
SMILES
C1=C(C=C(C(=C1[N+](=O)[O-])S(=O)(=O)O)[N+](=O)[O-])[N+](=O)[O-]
InChIKey
NHJVRSWLHSJWIN-UHFFFAOYSA-N
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Picrylsulfonic Acid (ca. 1% in N,N-Dimethylformamide) from TCI is a solution of 2,4,6-trinitrobenzenesulfonic acid, better known by its abbreviation TNBS, in DMF solvent at a concentration of approximately 1%. The product is made specifically for detecting primary amines. TNBS reacts with primary amino groups to form coloured trinitrophenyl derivatives, so amines can be seen by eye and also measured by spectrophotometry. In the laboratory, the reagent is used in thin-layer chromatography and in biochemical analysis, where it gives a simple and reliable way to show and measure amino groups.

The main advantage of this product is that it comes as a ready-to-use solution. Pure solid TNBS must be handled with great care because it is a polynitro compound. A dilute solution in DMF is therefore much more practical and safer for routine work. The TNBS reaction is fairly selective for primary amines, so it can tell them apart from many other functional groups. The coloured products give clear contrast on TLC plates and allow quantitative measurement with a UV-Vis spectrophotometer. Together these properties make it a dependable choice for both qualitative screening and quantitative work.

In Indonesia, this reagent is useful for biochemistry, pharmaceutical, food technology, and peptide synthesis laboratories. Researchers can use it to check for free amino groups in samples, follow reactions that involve amines, and support analytical methods in university research groups and industrial quality-control departments. Because it is supplied as a solution, laboratory staff do not need to prepare reagent solutions from hazardous solid material. This saves preparation time and supports consistent, repeatable results in teaching, research, and routine testing laboratories.

Related TCI products

  • Detection of primary amines: TNBS reacts with primary amino groups to form coloured trinitrophenyl derivatives, so the presence of amines can be confirmed visually in a simple and direct way.
  • Thin-layer chromatography visualisation: the coloured derivatives give clear contrast on TLC plates, which helps analysts find and identify amine-containing spots after separation.
  • Spectrophotometric quantification: the coloured reaction products can be measured with a UV-Vis spectrophotometer, so analysts can determine amino group content quantitatively.
  • Biochemical analysis: the reagent is used in biochemistry laboratories to study amino groups in biological samples, and its ready-to-use solution form supports routine and repeated testing.
  • Peptide synthesis monitoring: peptide chemists can use it to check for free primary amino groups during synthesis work, because the reaction is fairly selective for primary amines.

Brand TCI (product code P1447)
CAS number 2508-19-2
Molecular formula —
Purity —
Category Analytical Chemistry > Analytical Reagents > Chromatography Reagents and Solvents
Pack sizes as listed on this product page
Physical form solution, ca. 1% picrylsulfonic acid (TNBS) in N,N-Dimethylformamide
Storage keep the container tightly closed and follow the manufacturer's label and Safety Data Sheet

Store this reagent in its original, tightly closed container in a cool, dry, well-ventilated area, away from heat, ignition sources, and direct sunlight. Follow the specific storage conditions given on the TCI label and Safety Data Sheet. TNBS is a polynitro compound and DMF is a hazardous organic solvent, so handle the solution in a fume hood. Wear suitable gloves, safety goggles, and a laboratory coat, and avoid contact with skin and eyes and breathing in vapours. Do not let the solution evaporate to dryness, because solid TNBS needs very careful handling. Keep the reagent away from incompatible materials, and dispose of residues and contaminated materials according to local laboratory waste regulations.

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Analytical Chemistry

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