UV Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]

UV Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents] — includes aromatic acyl chlorides and isocyanates, such as benzoyl chloride, nitrobenzoyl chloride derivatives, and phenyl isocyanate, which react with hydroxyl groups to form chromophore-containing esters or carbamates. The nitro or substituted aromatic groups in these reagents enhance the UV light absorption of the resulting derivatives.

These reagents are used for derivatization of hydroxyl groups prior to high-performance liquid chromatography (HPLC) analysis, converting compounds with weak UV absorption into strongly chromophoric derivatives so they are easier to detect and quantify. Analytical chemists and quality control laboratories use them to improve detection sensitivity for sugars, alcohols, or other hydroxyl compounds in HPLC methods.

Example products in this collection: TCI B0105 98-88-4 Benzoyl Chloride, TCI I0131 103-71-9 Phenyl Isocyanate, TCI A5511 99-33-2 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling], TCI M0721 100-07-2 4-Methoxybenzoyl Chloride, TCI N0176 122-04-3 4-Nitrobenzoyl Chloride.

Choose a reagent based on the chromophore strength required, for example multiple nitro groups in dinitrobenzoyl chloride for higher sensitivity, as well as its reactivity toward the target hydroxyl group. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under HPLC Derivatization Reagents, often used together in one laboratory workflow:

Back to HPLC Derivatization Reagents. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — official distributor of TCI Japan in Indonesia — to check availability and lead time directly from Japan.

4 products