3,5-Dinitrobenzoyl Chloride [for HPLC Labeling] TCI A5511

Chemical Identity

CAS No.: 99-33-2
PubChem: CID 7432·SID 87563158
Formula: C7H3ClN2O5
Molecular weight: 230.56 g/mol
Purity: >99.0%(T)
Reference databases: ChemSpider·ECHA·Wikipedia

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 3,5-dinitrobenzoyl chloride
SMILES: C1=C(C=C(C=C1[N+](=O)[O-])[N+](=O)[O-])C(=O)Cl
InChIKey: NNOHXABAQAGKRZ-UHFFFAOYSA-N

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3,5-Dinitrobenzoyl Chloride is a chemical compound widely used in analytical chemistry for derivatization processes. It plays a crucial role in laboratories by enabling the modification of target molecules to enhance their detectability through advanced analytical techniques such as HPLC. This reagent is essential for improving the sensitivity and accuracy of chemical analysis, making it a valuable tool in both research and quality control settings. Its application is particularly relevant in environments where precise and reliable data are required for scientific investigations.

The compound is known for its chemical stability and reactivity towards various organic compounds. It exhibits strong affinity for functional groups such as amines, hydroxyls, and other reactive moieties, allowing it to form derivatives with enhanced detectability. These properties make it a preferred choice for analysts seeking reliable and consistent results. Its effectiveness in creating detectable derivatives ensures that it remains a key component in analytical workflows, especially in complex sample matrices where traditional detection methods may fall short.

In Indonesian laboratories, 3,5-Dinitrobenzoyl Chloride is commonly used in scientific research, product quality testing, and environmental analysis. Its ability to improve detection limits and provide clear analytical outcomes supports a wide range of applications. This reagent is particularly favored in settings where high precision and accuracy are critical, such as in academic and industrial research facilities across Indonesia.

Advantages (TCI brochure)

Reacts with a hydroxy group to form an ester or with an amino group to form an amide
Resulting ester or amide is stable and reaches the detector without decomposition in reversed phase HPLC
Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

HPLC Labeling Reagents 2026-03-10 · p. 2

Related TCI products

Used in HPLC labeling for enhanced detection of amino compounds due to its strong reactivity with amine groups.
Applied in environmental analysis to detect trace organic pollutants through improved chromatographic resolution.
Utilized in pharmaceutical research for the derivatization of drug compounds to facilitate quantification in complex mixtures.
Employed in food safety testing to identify and quantify amino acids in food samples with high sensitivity.
Integrated into analytical workflows for the identification of hydroxyl-containing compounds in chemical and biological samples.

From the TCI brochure

Alcohols (as 3,5-dinitrobenzoic acid esters)
Mono- and diethylene glycols in polyethylene glycol
Aliphatic alcohols

Brand	TCI
CAS number	99-33-2
Molecular formula	—
Purity	—
Category	Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes	Available in various quantities as per standard laboratory reagent packaging
Physical form	Solid, crystalline powder
Storage	Store in a cool, dry place away from light and moisture

Data from the TCI brochure

Panjang gelombang deteksi UV	254 nm (Kolom Kaseisorb LC ODS-300-5 4.6 mm I.D.x150 mm; CH3CN/H2O = 55/45; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

3,5-Dinitrobenzoyl Chloride should be stored in a cool, dry environment, away from direct sunlight and moisture. It is recommended to keep the compound in a sealed container to prevent exposure to air and humidity. Due to its reactivity, it should be handled in a well-ventilated area, preferably with appropriate personal protective equipment such as gloves and safety goggles. The material is not classified as hazardous under standard laboratory conditions, but care should be taken to avoid inhalation or skin contact. It is suitable for storage in glass or polyethylene containers, ensuring long-term stability and maintaining its chemical integrity. Always follow standard laboratory safety protocols when handling this reagent to ensure safe and effective use.

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