Nalpha-(5-Fluoro-2,4-dinitrophenyl)-D-leucinamide is a specialized chemical reagent used in the quantitative analysis of enantiomers in optically active compounds. This reagent plays a critical role in analytical chemistry, particularly in the field of chiral analysis. It is widely utilized in High-Performance Liquid Chromatography (HPLC) to determine the enantiomeric excess (e.e.) of a compound. Its ability to react with amino acid compounds and form detectable complexes makes it an essential tool for enantiomeric separation and identification.
The reagent is preferred for its chemical stability under specific conditions and its specificity in reacting with target compounds. Its complex molecular structure ensures sensitive and accurate detection in chromatographic analysis. This makes it ideal for applications requiring high precision and reliability. Additionally, its solid form facilitates easy handling and minimizes contamination risks during preparation, enhancing its usability in laboratory settings.
In Indonesian laboratories, this reagent is commonly used in pharmaceutical and chemical research. It is particularly valuable in drug quality testing and the analysis of chiral compounds. Its role in clarifying enantiomer separation is crucial for ensuring the accuracy of analytical results. The reagent's reliability and effectiveness make it a preferred choice for researchers and analysts working with optically active substances.
Advantages (TCI brochure)
- Reacts easily with amino groups for optical purity determination
- Racemic amino acids form diastereomers that are separated efficiently by reversed phase HPLC
- Absolute configuration of amino acids can be determined; high sensitivity by LC-MS
TCI brochures (PDF)
- HPLC Labeling Reagents 2026-03-10 · p. 2
Related TCI products
- TCI A5523 178065-29-7 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination]
- TCI D2259 95713-52-3 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]
- TCI A5522 68123-33-1 N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]
- TCI A5514 14152-97-7 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
- TCI A5512 70-34-8 2,4-Dinitrofluorobenzene [for HPLC Labeling]
- TCI A5511 99-33-2 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling]
- Enantiomer Analysis in Pharmaceutical Research: This reagent is ideal for determining enantiomeric purity in drug development, ensuring compliance with regulatory standards.
- Chiral Compound Identification: Its specificity allows for the precise detection and separation of chiral compounds, enhancing analytical accuracy.
- HPLC Method Development: It supports the creation of robust HPLC methods for enantiomeric analysis, improving the efficiency of chromatographic processes.
- Quality Control in Chemical Synthesis: It aids in verifying the purity of synthesized compounds, ensuring consistency and reliability in chemical production.
- Research in Organic Chemistry: Its utility in studying chiral interactions makes it a valuable tool for academic and industrial research.
From the TCI brochure
- Amino acids (as D-FDLA derivatives)
| Brand | TCI |
|---|---|
| CAS number | 178065-30-0 |
| Molecular formula | — |
| Purity | — |
| Category | Analytical Chemistry > Analytical Reagents > Derivatization Reagents |
| Pack sizes | As per supplier specifications |
| Physical form | Solid |
| Storage | Store in a cool, dry place away from light |
Data from the TCI brochure
| Batas deteksi | 5 pmol (ESI LC-MS; kolom Kaseisorb LC ODS 2000 2.0 mm I.D.x150 mm; 0.2 mL/min; 40 C) |
|---|
Source: TCI brochure A1096 (2026-03-10)
This reagent should be stored in a cool, dry environment, away from direct sunlight and moisture. It is recommended to use airtight containers to prevent contamination and maintain its chemical integrity. Due to its reactivity, it should be handled with care using appropriate personal protective equipment. Proper labeling of storage containers is essential to ensure safe handling. The reagent should not be exposed to incompatible substances or extreme temperatures. Regular inspection of storage conditions is advised to ensure optimal performance and safety in laboratory settings.
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