• 178065-29-7 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination] - AMI Scientific
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TCI

CAS 178065-29-7

Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination] TCI A5523

Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination] TCI A5523

Chemical Identity

CAS No.
178065-29-7
PubChem
CID 15817226·SID 87563164
Formula
C12H15FN4O5
Molecular weight
314.27 g/mol
Purity
>98.0%(HPLC)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
(2S)-2-(5-fluoro-2,4-dinitroanilino)-4-methylpentanamide
SMILES
CC(C)C[C@@H](C(=O)N)NC1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])F
InChIKey
WCOZOJGXDVGGIK-VIFPVBQESA-N
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TCI A5523 is a derivatization reagent used in analytical chemistry for determining the enantiomeric excess (e.e.) of optically active compounds. This reagent plays a crucial role in high-performance liquid chromatography (HPLC) labeling processes, where it reacts with target compounds to form detectable derivatives. Its application is essential in analytical laboratories, particularly in the study of chiral molecules such as amino acids and pharmaceuticals. The reagent enables precise and reliable analysis by enhancing the detectability of enantiomers through chemical modification.

The chemical properties of TCI A5523 make it a preferred choice for analytical applications. It exhibits high chemical stability and specificity, ensuring effective reaction with target compounds without altering their original molecular structure. The reagent is also known for its high efficiency in derivatization, delivering consistent and accurate analytical results. Its inert nature towards other chemical substances minimizes the risk of cross-contamination, making it a reliable and safe option for use in laboratory settings.

In Indonesian laboratories, TCI A5523 is commonly used in scientific research and pharmaceutical industries. It supports various studies involving enantiomeric analysis, quality testing, and the development of new products. Many educational institutions and research organizations rely on this reagent for its precision and reliability in analytical processes, contributing to the advancement of chemical and pharmaceutical sciences.

Advantages (TCI brochure)

  • Reacts easily with amino groups for optical purity determination
  • Racemic amino acids form diastereomers that are separated efficiently by reversed phase HPLC
  • Absolute configuration of amino acids can be determined; high sensitivity by LC-MS

TCI brochures (PDF)

Related TCI products

  • Enantiomeric Analysis in Pharmaceutical Research: This reagent is ideal for identifying and quantifying enantiomers in drug development, ensuring the purity and efficacy of chiral compounds.
  • Quality Control in Pharmaceutical Manufacturing: It supports the analysis of chiral impurities, helping to maintain product quality and compliance with regulatory standards.
  • Academic Research in Analytical Chemistry: TCI A5523 is widely used in universities and research centers for studying the optical properties of amino acids and other chiral molecules.
  • Forensic and Environmental Analysis: The reagent aids in the detection of chiral pollutants and substances in environmental and forensic samples.
  • Drug Metabolite Profiling: It facilitates the analysis of chiral metabolites, providing insights into drug metabolism and pharmacokinetics.

From the TCI brochure

  • Amino acids (as L-FDLA derivatives)

Brand TCI
CAS number 178065-29-7
Molecular formula —
Purity —
Category Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes —
Physical form Solid
Storage Store in a cool, dry place away from direct sunlight

Data from the TCI brochure

Batas deteksi5 pmol (ESI LC-MS; kolom Kaseisorb LC ODS 2000 2.0 mm I.D.x150 mm; 0.2 mL/min; 40 C)

Source: TCI brochure A1096 (2026-03-10)

TCI A5523 should be stored in a cool, dry location, away from direct sunlight and sources of heat. It is recommended to keep the reagent in a sealed container to prevent moisture absorption and contamination. Due to its chemical stability, it does not require refrigeration but should be protected from environmental factors that may affect its integrity. In laboratory settings, it is important to handle the reagent with appropriate personal protective equipment, such as gloves and safety goggles, to ensure safe usage. Always ensure that the reagent is stored in a well-ventilated area to minimize exposure to vapors. Proper labeling and storage practices help maintain the reagent's effectiveness and safety during use.

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Analytical Chemistry

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