• 68123-33-1 N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling] - AMI Scientific
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TCI

CAS 68123-33-1

N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling] TCI A5522

N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling] TCI A5522

Chemical Identity

CAS No.
68123-33-1
PubChem
CID 100796·SID 87563163
Formula
C12H10N2O6
Molecular weight
278.22 g/mol
Purity
>98.0%(N)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
(2,5-dioxopyrrolidin-1-yl) 2-(4-nitrophenyl)acetate
SMILES
C1CC(=O)N(C1=O)OC(=O)CC2=CC=C(C=C2)[N+](=O)[O-]
InChIKey
JUEAHIVORWVODA-UHFFFAOYSA-N
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TCI A5522 68123-33-1 N-Succinimidyl 4-Nitrophenylacetate is a chemical reagent used in derivatization processes for high-performance liquid chromatography (HPLC) analysis. It serves as a labeling agent for organic compounds, enabling the detection and identification of components in samples through UV spectral changes. This reagent plays a crucial role in enhancing the sensitivity and accuracy of quantitative analysis, especially for compounds with low UV response. Its utility lies in its ability to form stable bonds with a wide range of organic molecules, making it a versatile tool in analytical chemistry.

The chemical and reactive properties of N-Succinimidyl 4-Nitrophenylacetate make it an ideal choice for derivatization reactions. Its stable and reactive structure allows it to efficiently label target analytes, improving their detectability in chromatographic systems. The reagent’s non-toxic nature and strong reactivity contribute to its popularity in laboratory settings. Its consistent performance and reliability ensure accurate and reproducible results, making it a preferred option for analytical chemists.

In Indonesian laboratories, this reagent is highly relevant for research institutions, universities, and companies engaged in chemical analysis. With the demand for accuracy and precision in testing, this reagent is a primary choice for improving the quality of analytical data. Its application in HPLC labeling supports the advancement of analytical techniques in various scientific fields.

Advantages (TCI brochure)

  • Succinimidyl group reacts easily with an amino group to form the corresponding amide
  • Resulting amide is stable enough to reach the detector in reversed phase HPLC
  • Excellent chromatogram obtained at 254 nm, a wavelength generally used for UV detection

TCI brochures (PDF)

Related TCI products

  • High-Performance Liquid Chromatography (HPLC) analysis benefits from this reagent's ability to enhance UV detection of low-response compounds, improving sensitivity and accuracy in complex sample analysis.
  • Organic compound identification in environmental and pharmaceutical research relies on its capacity to form stable bonds with various molecular structures, enabling clear spectral differentiation.
  • Quantitative analysis in food and drug testing utilizes its consistent reactivity to ensure reliable and reproducible results, supporting regulatory compliance and quality control.
  • Biochemical studies benefit from its non-toxic properties and stability, allowing safe and effective labeling of biomolecules for detection and structural analysis.
  • Analytical method development in academic and industrial laboratories uses its versatility to optimize detection protocols for a wide range of organic compounds.

From the TCI brochure

  • Alkylamines (as 4-nitrophenylacetamides)
  • Drugs (amphetamine, methamphetamine)

Brand TCI
CAS number 68123-33-1
Molecular formula —
Purity —
Category Analytical Chemistry > Analytical Reagents > Derivatization Reagents
Pack sizes Available in standard laboratory quantities as per supplier specifications
Physical form Solid or liquid, depending on the specific product formulation
Storage Store in a cool, dry place away from direct sunlight and moisture

Data from the TCI brochure

Panjang gelombang deteksi UV254 nm (Kolom Kaseisorb LC ODS-100-5 4.6 mm I.D.x150 mm; CH3OH/H2O = 60/40; 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

This reagent should be stored in a cool, dry environment, away from direct sunlight and moisture to maintain its stability and reactivity. It is recommended to use airtight containers to prevent exposure to air and humidity, which could affect its performance. Proper labeling of storage containers is essential to ensure safe handling and prevent cross-contamination. In laboratory settings, it is important to follow standard safety protocols, including the use of personal protective equipment when handling. Regular monitoring of storage conditions ensures the reagent remains effective for analytical applications. Always follow manufacturer guidelines for safe storage and use.

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Product Categories

Analytical Chemistry

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