Collection: Fluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]

Fluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents] — includes benzoxadiazole and maleimide derivatization reagents that react selectively with free thiol groups, forming fluorescent derivatives for the detection and quantification of thiol-containing compounds and cysteine residues in HPLC-based protein analysis.

Reagents such as ABD-F, DBD-F, and NBD-F in this category are used by biochemistry researchers to label thiol groups on amino acids, peptides, or proteins before separation and fluorescence detection by HPLC, while maleimide derivatives such as N-(1-pyrenyl)maleimide are used to label cysteine residues in proteomics studies. The chiral isothiocyanate reagents (R)- and (S)-DBD-Py-NCS enable the separation and configuration determination of enantiomeric amino acids in chromatographic analysis.

Product examples in this collection: TCI A5597 91366-65-3 ABD-F [=4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for Determination of Thiols], TCI P1214 42189-56-0 N-(1-Pyrenyl)maleimide, TCI A5596 664985-43-7 DAABD-Cl [=4-[2-(Dimethylamino)ethylaminosulfonyl]-7-chloro-2,1,3-benzoxadiazole] [for Proteome Analysis], TCI A5558 156153-43-4 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling], TCI A5595 98358-90-8 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling], TCI A5568 163927-31-9 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling], TCI A5593 29270-56-2 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling], TCI A5569 163927-32-0 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling].

Pay attention to the excitation and emission wavelengths of the fluorophore, the reactive group (sulfonyl fluoride, acid chloride, or maleimide) suited to the thiol target, and the enantiomeric purity for chiral reagents such as DBD-Py-NCS. All products are genuine Tokyo Chemical Industry (TCI) Japan products, with purity, packaging, and storage conditions listed on each item's page.

Other categories at the same level under HPLC Derivatization Reagents, often used together in one laboratory workflow:

• Fluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
• UV Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Carboxy Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]
• UV Detection Labeling Reagents (for Carboxyl Groups) [HPLC Derivatization Reagents]
• Fluorescence Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]

This collection is further divided into 1 more specific group:

• Protein Analysis Reagents

Back to HPLC Derivatization Reagents. For large-volume procurement, special packaging, or specific grades, contact the AMI Scientific team — the official TCI Japan distributor in Indonesia — to check availability and lead time directly from Japan.