Dansylamino-PITC [Fluorescent Coupling Reagent for Edman Degradation] TCI A5354

Chemical Identity

CAS No.: 102417-94-7
PubChem: CID 128100·SID 87563147
Formula: C19H17N3O2S2
Molecular weight: 383.5 g/mol
Purity: >98.0%(HPLC)(N)

Full identifiers (IUPAC, SMILES, InChIKey)

IUPAC: 5-(dimethylamino)-N-(4-isothiocyanatophenyl)naphthalene-1-sulfonamide
SMILES: CN(C)C1=CC=CC2=C1C=CC=C2S(=O)(=O)NC3=CC=C(C=C3)N=C=S
InChIKey: MZGXHCHKGRQLHR-UHFFFAOYSA-N

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↓ Unduh SDS / MSDS (PDF resmi TCI)

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Dansylamino-PITC (102417-94-7) is a fluorescent coupling reagent widely used in the Edman degradation process, a fundamental technique in protein and peptide analysis. This reagent plays a critical role in identifying the amino acid sequence at the N-terminal end of proteins, enabling researchers to determine the structure of proteins with high accuracy. In laboratory settings, it is essential for biochemical studies that require precise and sensitive detection methods. Its application is crucial in both academic and industrial research, supporting advancements in biochemistry and molecular biology.

The strong fluorescent properties of Dansylamino-PITC make it a preferred choice for analytical techniques such as spectroscopy and chromatography. Its fluorescence allows for easy detection of reaction products, enhancing the sensitivity and reliability of results. The reagent is stable under standard laboratory conditions, ensuring consistent performance across various experiments. These characteristics make it a reliable tool for researchers aiming to achieve high-quality data in protein analysis.

In Indonesian laboratories, Dansylamino-PITC is commonly used in biomedical research, pharmacology, and biotechnology. It is particularly valuable in institutions conducting protein structure studies and peptide analysis. Its fluorescent properties and stability make it an essential component in the analytical toolkit of Indonesian research facilities, supporting both basic and applied scientific investigations.

Advantages (TCI brochure)

Green-yellow fluorescence from dansyl core, dependent on environment

TCI brochures (PDF)

Fluorescent Labeling Dyes 2025-11-18 · p. 2
Reagents for Biological Research 2025-03-11 · p. 4

Related TCI products

Edman Degradation is a method for determining the amino acid sequence of proteins, and Dansylamino-PITC is ideal for this due to its strong fluorescence and coupling efficiency.
Peptide Analysis requires high sensitivity, and the fluorescent properties of this reagent enhance detection accuracy in chromatographic and spectroscopic methods.
Protein Structure Studies benefit from the reagent’s ability to identify N-terminal amino acids, providing crucial data for structural and functional analysis.
Biomedical Research often involves protein characterization, and this reagent supports such studies with its reliability and sensitivity in analytical techniques.
Biotecnological Applications require precise analytical tools, and the stability and fluorescence of Dansylamino-PITC make it suitable for various biotech research projects.

From the TCI brochure

Can be labeled to amino groups by thiourea bond formation

Brand	TCI
CAS number	102417-94-7
Molecular formula	—
Purity	—
Category	Life Science > Biochemicals and Reagents > Fluorescent Probes, Fluorescent Labels, Fluorescent Stains
Pack sizes	—
Physical form	—
Storage	Store in a cool, dry place away from light

Data from the TCI brochure

λex,max	ca. 335 nm
λem,max	ca. 460 nm

Source: TCI brochure LL039 (2025-11-18)

Dansylamino-PITC should be stored in a cool, dry place away from direct light to maintain its stability and fluorescent properties. It is recommended to use airtight containers to prevent moisture exposure, which could affect its performance. General laboratory precautions include handling with care to avoid contamination and ensuring proper ventilation when working with the reagent. The reagent is typically used in controlled environments where precise analytical techniques are required. Proper storage and handling ensure consistent results and prolong the reagent’s usability in various research applications.

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