• 131467-87-3 NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling] - AMI Scientific
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TCI

CAS 131467-87-3

NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling] TCI A5557

NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling] TCI A5557

Chemical Identity

Other names
NBD-H
CAS No.
131467-87-3
PubChem
CID 16218437·SID 87563175
Formula
C6H9N7O3
Molecular weight
227.18 g/mol
Purity
>98.0%(HPLC)
Full identifiers (IUPAC, SMILES, InChIKey)
IUPAC
hydrazine;(4-nitro-2,1,3-benzoxadiazol-7-yl)hydrazine
SMILES
C1=C(C2=NON=C2C(=C1)[N+](=O)[O-])NN.NN
InChIKey
BLIOAKMSUDUCKB-UHFFFAOYSA-N
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NBD-H (4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) is a chemical compound widely used in laboratory settings for quantitative and qualitative analysis. It serves as a key reagent in derivatization processes, particularly for labeling in High-Performance Liquid Chromatography (HPLC). This compound enhances the fluorescent properties of target molecules, making them more detectable and identifiable in chromatographic systems. Its role is crucial in analytical chemistry, especially in applications requiring high sensitivity and specificity.

NBD-H is valued for its chemical stability under specific conditions and its ability to react with a variety of molecules, particularly those containing nitrogen and carbon. These properties make it a preferred choice for chemical modification of analytes in analytical applications. Its reactivity and specificity allow for accurate and reliable results in complex sample analyses. The compound's versatility supports its use across multiple analytical techniques, making it a reliable tool for researchers and laboratories.

In Indonesian laboratories, NBD-H is commonly used in scientific research, pharmaceutical development, and quality control processes. Its application is essential in scenarios where sensitive and specific detection is required. The compound's reliability and effectiveness have made it a standard reagent in analytical workflows, supporting both academic and industrial research efforts.

Advantages (TCI brochure)

  • Reagent itself non-fluorescent; hydrazones after reaction have strong fluorescence
  • Resultant hydrazone stable under reversed phase HPLC
  • Longer wavelengths give less interference; highly sensitive due to high reactivity

TCI brochures (PDF)

Related TCI products

  • HPLC Labeling – NBD-H is ideal for labeling compounds in HPLC due to its ability to enhance fluorescence, enabling precise detection and identification of analytes.
  • Derivatization Reactions – Its reactivity with nitrogen and carbon-containing compounds makes it suitable for modifying analytes to improve detection sensitivity.
  • Quantitative Analysis – NBD-H supports accurate quantification by increasing the fluorescence of target molecules, facilitating reliable data collection.
  • Pharmaceutical Research – It is used in drug development to analyze metabolites and compounds, ensuring the accuracy of chemical profiling.
  • Quality Control Testing – NBD-H aids in detecting trace amounts of substances in samples, supporting rigorous quality assurance in industrial and research settings.

From the TCI brochure

  • Aldehydes or ketones

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  • Brand – TCI
  • CAS Number – 131467-87-3
  • Category – Analytical Chemistry > Analytical Reagents > Derivatization Reagents
  • Pack Sizes – As per manufacturer specifications
  • Physical Form – Solid (as per standard product description)
  • Storage Note – Store in a cool, dry place away from light and moisture

Data from the TCI brochure

Excitation wavelength470 nm (Fluorescence detection)
Emission wavelength550 nm (Fluorescence detection)
Detection limit35 fmol (Propionaldehyde)
Mobile phaseCH3CN / 0.05% TFA in H2O = 50 / 50 (Propionaldehyde, 30 C, Kaseisorb LC ODS Super, 1 mL/min)

Source: TCI brochure A1096 (2026-03-10)

NBD-H should be stored in a cool, dry environment, away from direct light and moisture to maintain its chemical stability. It is recommended to use airtight containers to prevent exposure to air and humidity, which could affect its reactivity. Proper labeling of storage containers is essential to ensure safe handling. In laboratory settings, personal protective equipment such as gloves and safety goggles should be worn when handling the compound. It is important to follow standard safety protocols to minimize any potential risks associated with its chemical properties. Regular inspection of storage conditions ensures the compound remains effective for its intended analytical applications.

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